量子电子学报2016,Vol.33Issue(4):437-443,7.DOI:10.3969/j.issn.1007-5461.2016.04.008
全场光学相干层析在细胞成像中的应用研究
Application of full-field optical coherence tomography for cell imaging
摘要
Abstract
A Linnik type full-field optical coherence tomography (FF-OCT) system is developed for tomographic imaging of biological cells.Different from other imaging methods used in FF-OCT systems,an image restoration method based on Hilbert transform is adopted,which requires only two interference images to recover the structure information of the sample.Even if the phase shift error occurs,the sample chromatogram can still be obtained.The measured longitudinal and lateral resolutions of the system reach 1.1 μm and 2.5 μm,respectively.Feasibility of the FF-OCT system is verified by preliminary imaging experiments of onion epidermal cells,and it lays foundation for the further realization of high resolution cell imaging.关键词
图像处理/显微/全场光学相干层析/成像系统/希尔伯特变换Key words
image processing/microscopy/full-field optical coherence tomography/imaging system/Hilbert transform分类
信息技术与安全科学引用本文复制引用
胡斌,江兴方,石晓灏,刘磊,邓林红..全场光学相干层析在细胞成像中的应用研究[J].量子电子学报,2016,33(4):437-443,7.基金项目
Supported by National Natural Science Foundation of China-Program for Young Scientists(国家自然科学基金青年基金,11402037),Changzhou Key Laboratory of Respiratory Medical Engineering(常州市呼吸医学工程重点实验室项目,CM20133005) (国家自然科学基金青年基金,11402037)