特产研究2016，Vol.38Issue(3)：25-30,6.DOI:10.16720/j.cnki.tcyj.2016.03.006
HPLC －ESI－MS法在西洋参总皂苷提取工艺研究中的应用
Optimization of Extraction Process of Ginsenosides from Panax quinquefolium L .Using HPLC-ESI-MS
摘要
Abstract
To optimize extraction process of ginsenosides from Panax quinquefolium L .UV was applied to determine the content of total saponins with ginsenoside Re as index component .The influences of methonal amout ,extraction time and the times of methonal and water -saturated n-butanol extractions on total ginsenosides contents were investigated by orthogonal test .The soxhlet extraction of P .quinquefolium L .was opti-mized .Compare the contents of total saponins using heating reflux extraction ,ultrasonic extraction and soxhlet extraction by HPLC -ESI-MS , the soxhlet extraction result is best .The optimal extraction conditions were as follows :soxhlet extraction process ,extracted three times with two siphon quantities at 87℃ and 1h for each time followed by three times of water -saturated n-butanol extraction .This optimized extraction process is of high extraction rate ,effective and high purity of ginsenosides ,could provide a reference for development and utilization of P .quin-quefolium L .关键词
西洋参/总皂苷/提取方法/紫外-可见分光光度法/液相色谱-质谱联用Key words
Panax quinquefolium L ./ginsenosides/extraction process/UV/HPLC-ESI-MS分类
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白雪,黄鑫,李帅坪,刘淑莹..HPLC －ESI－MS法在西洋参总皂苷提取工艺研究中的应用[J].特产研究,2016,38(3):25-30,6.基金项目
吉林省科技发展计划项目（20160520181JH）；吉林省教育厅“十三五”科学技术研究项目（吉教科合字［2016］第30号）；公益性行业（农业）科研专项经费项目 （）
