特产研究2016，Vol.38Issue(3)：31-35,5.DOI:10.16720/j.cnki.tcyj.2016.03.007
应用 TTC 法快速测定北细辛种子生活力
Study on TTC Method for Testing Seed Viability of Asarum heterotropoides Fr .Schmidt var .mandshuricum (Maxim .)Kitag
摘要
Abstract
The aim of the study is to study an appropriate method for testing seed viability of Asarum heterotropoides Fr .Schmidt var .mand-shuricum (Maxim .)Kitag .Using the 2 ,3 ,5-triphenyl tetrazolium chloride (TTC ) method ,the orthogonal test design set different soaking time , different TTC concentration ,different dyeing temperature ,and different dyeing time were detected to research seed viability measurement condi-tions .The optimum conditions for testing seed viability were soaking seeds for 6h and then staining in 0.1% TTC solution at 30℃ for 18h .Un-der these conditions seed viability is significantly related to germination .Research the optimum conditions for testing seed viability ,It has great significance in cultivation and germplasm conservation .TTC method could be used as the standard method for testing seed viability .关键词
北细辛/2,3,5-氯化三苯基四氮唑法/种子生活力Key words
Asarum heterotropoides F .Schmidt var .mandshuricum (Maxim .) Kitag ./TTC Method/seed viability分类
农业科技引用本文复制引用
邢丽伟,于营,欧阳艳飞,张新静,朴向民,张佳琪,郭靖..应用 TTC 法快速测定北细辛种子生活力[J].特产研究,2016,38(3):31-35,5.基金项目
国家科技重大专项（2009ZX09308、2012ZX09304006）；国家中医药管理局国家公共卫生专项；吉林省质量技术监督局项目 （）
