波谱学杂志2016，Vol.33Issue(3)：442-451,10.DOI:10.11938/cjmr20160309
定量核磁共振法同时测定复合维生素B片中B1、B2、烟酰胺和泛酸钙
A Quantitative NMR Method to Analyze Vitamin B1, Vitamin B2, Nicotinamide and Calcium Pantothenate Contents in Compound Vitamin B Tablets
摘要
Abstract
A quantitative1H NMR method was established for analysis of contents of vitamin B1, vitamin B2, nicotinamide and calcium pantothenate in Compound Vitamin B Tablets. The experimental settings were as following: delay time (D1) 1.0 s, pulse width (P1) 3.0μs, number of sampling (NS) 500 times, 1,4-benzenedialdehyde peak atδ10.12 (2H) as the internal standard, and deuterated DMSO-d6 as the solvent. The peaks for quantitative analysis were: vitamin B1 atδ9.60~9.90 (1H), vitamin B2 atδ11.36 (1H), nicotinamide atδ9.01 (1H) and calcium pantothenate atδ0.78 (12H). The slopes of the standard curves obtained for the 4 components were close to theoretically predicted values. Calibration with vitamin B1 and calcium pantothenate reference substances yielded correlation coefficients (r) above 0.999 and recovery ratio near 100%. Finally, the method was applied to 4 real samples, and the results suggested that it is simple to use, accurate and reliable.关键词
定量核磁共振氢谱(1H qNMR)/复合维生素B片/维生素B1(VB1)/维生素B2(VB2)/烟酰胺/泛酸钙Key words
quantitative1H NMR/Compound Vitamin B Tablets/vitamin B1 (VB1)/vitamin B2 (VB2)/nicotinamide/calcium pantothenate分类
数理科学引用本文复制引用
郭强胜,刘明珂,禹珊,沈婷婷,宋巍,许旭..定量核磁共振法同时测定复合维生素B片中B1、B2、烟酰胺和泛酸钙[J].波谱学杂志,2016,33(3):442-451,10.基金项目
上海市自然科学基金资助项目(15ZR1440800) （15ZR1440800）
