高等学校化学学报2016，Vol.37Issue(9)：1622-1628,7.DOI:10.7503/cjcu20160147
花生致敏糖蛋白Ara h1糖链决定簇的质谱分析
Analysis of Antigenic Determinant Glycans of Peanut Allergy Glycoprotein Ara h1 by Mass Spectrometry
摘要
Abstract
Peanut seed total protein and glycoprotein Ara h1, which can cause allergic reactions, were chosen as the target materials in this study. Firstly, the glycans were released and labeled with 1-phenyl-3-methyl-5-pyrazolone( PMP) by recently developed “one-pot” method. Subsequently, the glycans were fractionated and enriched through C18 solid phase extraction column. Finally, the obtained glycans were analysed by electros-pray ionization mass spectrometry ( ESI-MS), MSn and Hydrophilic interaction liquid chsomatography-mass spectrometry( HILIC-MS) . The results indicated that Ara h1 has ten N-glycans, including seven high-mannose type, two ones with xylose modification, and one allergenic core α1,3-fucosylated N-glycan, the content of which is 12. 45% of total glycans.关键词
花生过敏/致敏糖蛋白Ara h1/糖链结构/电喷雾质谱/亲水性液相色谱-质谱联用Key words
Peanut allergy/Allergenic glycoprotein Ara h1/Glycan structure/Electrospray ionization mass spectrometry( ESI-MS)/Hydrophilic interaction liquid chsomatography-mass spectrometry( HILIC-MS)分类
化学化工引用本文复制引用
彭艺芳,王仲孚,王承健,王晶晶,李玲梅,晋万军,强珊,师红丹,张英,黄琳娟..花生致敏糖蛋白Ara h1糖链决定簇的质谱分析[J].高等学校化学学报,2016,37(9):1622-1628,7.基金项目
国家自然科学基金(批准号:21375103,31370804)资助. Supported by the National Natural Science Foundation of China(Nos.21375103,31370804) （批准号:21375103,31370804）
