广西植物2016，Vol.36Issue(8)：943-948,6.DOI:10.11931/guihaia.gxzw201411038
锐药竹的种子性状及分子遗传变异
Seed characters and genetic variation of Oxytenanthera abyssinica
摘要
Abstract
Oxytenanthera abyssinica is the most important indigenous bamboo in Africa. It plays an important role in local life, production and environment protection. However, the study of its genetic variation is little. We studied seed charac-ters and its variation, as well as the genetic variation of its seedlings abide by GB2772-1999. The length of seeds was 18.2 mm, width was 3.3 mm, 1 000 seed mass was 117.5 g and germination percentage was 66.5%. The results showed that little variation occurred in its seed characters, and variation degree was similar to that of other bamboos. More than 15 000 seedlings was cultured, leaves of 64 individuals were chosen as test material typically, 39 of them were chosen randomly and 25 individuals with big leaves. AFLP was applied to test its genetic variation, eight pair of primers are used. After electrophoretic separation, 1 728 lines was calculated by GeneScan 3. 1. It occurred that its PPB was 90.28%, Nei’ s gene diversity index was 0.243 6, Shannon index was 0.362 3. The result showed that the genetic varia-tions was abundant in O. abyssinica. Based on simple matching coefficient(SM), the individuals were clustered by UPG-MA cluster analysis. It indicated that the SM index ranged from 0.73 to 0.85. The samples can be clustered into three classes when SM similarity coefficient is 0.76. Combine phenotypic and physiological indicators systematic germplasm ex-aviation is further needed. This study will provide scientific information for biodiversity breeding and improved breeds.关键词
非洲竹区/种子性状/种子批/AFLP/种质资源Key words
African bamboo area/seed characters/seed lot/AFLP/germplasm resource分类
农业科技引用本文复制引用
廉超,张国武,冯云,冉洪,张莹,郭起荣..锐药竹的种子性状及分子遗传变异[J].广西植物,2016,36(8):943-948,6.基金项目
国家“948”项目(非洲重要特有经济竹子种质资源引进)(2012-4-48)[Supported by the National Program of Introducing Advanced Agri-cultural Science and Technology from Africa (2012-4-48)]。 （非洲重要特有经济竹子种质资源引进）
