解放军医药杂志2016，Vol.28Issue(8)：15-18,22,5.DOI:10.3969/j.issn.2095-140X.2016.08.004
cAMP/PKA信号通路在肾上腺肿瘤中的作用
Effect of cAMP/PKA Signaling Pathways on Adrenal Tumors
张科 1刘昌荣1
作者信息
- 1. 443000 湖北 宜昌，宜昌市第一人民医院泌尿外科
- 折叠
摘要
Abstract
Objective To investigate effect of cyclic adenosine monophosphate/protein kinase a ( cAMP/PKA) signaling pathways on adrenal tumors. Methods Human H295R cells of adrenal tumor cells were randomly divided into control group, group A and B. Control group did not undergo any treatment;group A was incubated with cAMP deriva-tives db-cAMP;group B was incubated with db-cAMP and inhibitor of cAMP/PKA signaling pathway H-89. In three groups, PKA activities were detected, and phosphorylation levels of CREB protein were also detected by Western Blot method;72 h cell proliferation levels were detected by CCK8 assay, and capacities of hormone secretion were detected by glucocorticoid secretion experiment. Results Levels of PKA activity, CREB protein phosphorylation, cell proliferation and glucocorticoid secretion were significantly higher in group A than those in control group and group B (P<0. 05);there were no significant differences in the levels between group B and control group (P>0. 05). Conclusion The ab-normal activation of cAMP/PKA signaling pathways can promote formation and functional effect of adrenal tumors.关键词
肾上腺肿瘤/环磷酸腺苷/蛋白激酶AKey words
Adrenal gland neoplasms/Cyclic adenosine monophosphate/Protein kinase a分类
医药卫生引用本文复制引用
张科,刘昌荣..cAMP/PKA信号通路在肾上腺肿瘤中的作用[J].解放军医药杂志,2016,28(8):15-18,22,5.
