摘要
Abstract
Objective To study possible mechanism of reducing glucose-concentration in cerebrospinal fluid ( CSF) in premature infants with severe intraventricular hemorrhage ( IVH) . Methods Levels of CSF glucose-concen-tration in 17 premature infants with severe IVH were objectively described. Expressions of glucose transporter GLUT1 and GLUT3 proteins in brain tissue in IVH preterm rabbits were quantitatively analyzed using Western Blot. Results Aver-age CSF glucose-concentration in the 17 premature infants decreased, the obviously decreased value was found in 16-20 d after the bleeding, and then the value was gradually restored, but the value was still lower on 26-30 d after the bleeding than the normal range in the literature. In severe IVH model group, expressions of GLUT1 protein from high to low were at 24 h, 48 h and 72 h after birth in turn, and the differences were statistically significant between each two time point (P<0. 05). In severe IVH model group, at 72 h after birth, expression of GLUT1 protein was lower than that in control group (P<0. 01) at 24 and 48 h after birth, the expressions were higher than those in control group (P<0. 01); ex-pression differences of GLUT3 protein were statistically significant between each two time point ( P <0. 05 ) , and the highest expression was found at 48 h, and followed by 24 h and 72 h after birth. In severe IVH model group, at 24 h after birth, there was no significant difference in GLUT3 protein expression compared with that in control group (P>0. 05);at 48 h after birth, the expression was higher than that in control group (P<0. 01);at 72 h after birth, the expression was lower than that in control group (P<0. 01). Conclusion Expression of GLUT1 protein in brain of severe IVH pre-term rabbits has a temporary increase, and then it begins to decrease, which may possibly induce reducing of glucose con-centration in cerebrospinal fluid. Expression of GLUT3 protein in brain has a temporary increase, and then it begins to decrease, which may lead to inadequate supply of glucose, and therefore it suggests that it may be involved in secondary brain injury after severe germinal matrix-intraventricular hemorrhage ( GM-IVH) .关键词
基质-脑室内出血/颅内出血/早产儿/动物模型/兔/脑脊液/葡萄糖/葡萄糖转运蛋白Key words
Key words] Germinal matrix/-Intraventricular hemorrhage/Intracranial hemorrhage/Premature infant/Animal /model/Rabbilts/Cerebrospinal fluid/Glucose/Glucose transporters分类
医药卫生
