郑州大学学报（医学版）2016，Vol.51Issue(5)：572-575,4.DOI:10.13705/j.issn.1671-6825.2016.050.04
ErbB3／Her3基因重组慢病毒的构建及应用
Construction and application of recombinant ErbB 3/Her3 lentiviral
摘要
Abstract
Aim: To construct lentiviral Lenti-Her3 and identify ErbB3H/er3 function in p44 pathway.Methods:Her3-1 and Her3-2 fragments of ErbB3/Her3 gene were cloned in turn into pCDH-CMV-MCS-EF1-Puro to generate pCDH-Her3,and then was packaged to obtain Lenti-Her3.Lenti-Her3 was infected in A549 cells which had been transfected with p44-shRNA, and then expressions of P44 and Her3 proteins were detected by Western blot, cell growth was detected through cell counting.Results:The A549 cells transfected with p44-shRNA had no expression of P44 protein, low expres-sion of Her3 protein, and low cell number counting.The expression of Her3 protein in the A549 cells transfected with p44-shRNA and infected with Lenti-Her3 was improved, and cell number counting was increased, but did not reach the normal level.Conclusion:The lentiviral Lenti-Her3 has been successfully constructed;p44 could promote lung cancer cell growth partially by Her3 pathway, which may be a potential therapeutic target for lung cancer.关键词
慢病毒/Her3/p44Key words
lentiviral/Her3/p44分类
医药卫生引用本文复制引用
生秀梅,王正新..ErbB3／Her3基因重组慢病毒的构建及应用[J].郑州大学学报（医学版）,2016,51(5):572-575,4.基金项目
国家自然科学基金项目31000046；江苏大学高级人才启动基金11JD063；国家博士后基金面上项目2015M571702 （）
