郑州大学学报（医学版）2016，Vol.51Issue(5)：591-594,4.DOI:10.13705/j.issn.1671-6825.2016.05.009
NOX1 siRNA 对 TNF-α诱导的 A549细胞凋亡的影响
Influence of NOX1 siRNA on apoptosis of A549 cells induced by TNF-α
摘要
Abstract
Aim:To investigate the effect of NOX1 on apoptosis of A549 cells induced by TNF-α.Methods: A549 cells were allocated into 3 groups:blank control group,TNF-αgroup and TNF-α+NOX1 siRNA group.Cells in TNF-α+NOX1 siRNA group were transfected with NOX1 siRNA through the transient transfection technology for 12 h,then cultured with TNF-α(10 μg/L) for 48 h.Cells in blank control group and TNF-αgroup were only cultured with medium and TNF-α(10 μg/L) for 48 h,respectively.After culture, the apoptosis rate was detected by Annexin V-FITC and PI staining,the ROS level in cells was detected by DCFH fluorescent probe method, and the expressions of NOX1 and p-JNK protein were detected through Western blot.Results:Compared with blank control group,the apoptosis rate,ROS level and the expres-sions of NOX1 and p-JNK were increased in TNF-αgroup( P<0.05); while compared with TNF-αgroup, the apoptosis rate,ROS level and the expressions of NOX1 and p-JNK were decreased in TNF-α+NOX1 siRNA group(P<0.05).Con-clusion:NOX1 could increase ROS level, then active JNK/MAPK signal pathway, and induce A549 cell apoptosis.关键词
A549细胞/NOX1/TNF-α/凋亡Key words
A549 cell/NOX1/TNF-α/apoptosis分类
医药卫生引用本文复制引用
夏艳秋,王娜,阙菡雅,徐小艳,薛腾,燕贞,姚武,刘莹,周舫..NOX1 siRNA 对 TNF-α诱导的 A549细胞凋亡的影响[J].郑州大学学报（医学版）,2016,51(5):591-594,4.基金项目
河南省高等学校重点科研项目16A330008 （）
