郑州大学学报（医学版）2016，Vol.51Issue(5)：598-601,602,5.DOI:10.13705/j.issn.1671-6825.2016.05.011
EGCG对哺乳期铅暴露仔鼠学习记忆能力和海马抗氧化损伤作用的影响
Effects of EGCG on learning and memory performance and antioxidative injury ability in lead exposed offspring mice during lactation
摘要
Abstract
Aim:To explore the effects of epigallocatechin gallate( EGCG) on spatial learning memory performance and antioxidative damage ability in lead exposed offspring mice during lactation.Methods: A total of ten healthy preg-nant C5 BL/6 mice were randomly allocated into control group and lead exposed group, which were treated with drinking double evaporated water and Pb containing water, respectively from the beginning of offspring birth and lasted until wea-ning.There were 2 pregnant mice in control group and 8 pregnant mice in lead exposed group.The offspring mice were exposed to lead by maternal milk.Forty lead exposed offspring mice and ten control offspring mice were employed as the research objects.According to the body weight, the mice were stochastically allocated into five groups: lead exposed group( normal saline) ,low,middle,high dose EGCG interfering groups and control group( ten control offspring mice) and each group was administered for by lavage 21 d.The spatial learning and memory ability was evaluated by Morris water maze,SOD and GSH-Px activity levels and MDA level in hippocampus tissue were measured by corresponding kits.Re-sults: The escape latency in each group measured at different days had significant differences and there were also differ-ences between different groups( P<0.001);the activity levels of SOD and GSH-Px were higher in middle,high interfer-ing groups than those in lead exposed group, while the content of MDA was lower(P<0.05).Conclusion:EGCG may protect offspring mice from lead-induced peroxidation and enhance the spatial learning and memory ability through impro-ving antioxidant enzymes activity.关键词
表没食子儿茶素没食子酸酯/哺乳期铅暴露/仔鼠/学习记忆/抗氧化损伤Key words
epigallocatechin gallate/lead exposed during lactation/offspring mice/learning and memory/antioxidative injury分类
农业科技引用本文复制引用
赵林远,尹延彦,李静,张小欢,蒋鸿琳,李文杰..EGCG对哺乳期铅暴露仔鼠学习记忆能力和海马抗氧化损伤作用的影响[J].郑州大学学报（医学版）,2016,51(5):598-601,602,5.基金项目
国家自然科学基金资助项目81172716 （）
