郑州大学学报（医学版）2016，Vol.51Issue(5)：622-626,5.DOI:10.13705/j.issn.1671-6825.2016.05.017
人脑胶质瘤组织中 CPEB4的表达及与细胞迁移和侵袭的关系
Expression of CPEB4 in human brain glioma tissue and its relationship with cell invasion and migration
摘要
Abstract
Aim:To explore the expression of CPEB4 in human brain glioma tissue and its relationship with cell inva-sion and migration.Methods:A total of 30 paired fresh tissue were obtained from the resection specimens of glioma pa-tients.The expression of CPEB4 in tumor and adjacent normal tissue was detected by Western blot.CPEB4 expression was upregulated and downregulated by overexpressing plasmid and siRNA in glioma cell lines SKMG-4 and T98.The cell inva-sion and migration was detected by Transwell assay.MMP2 and MMP9 expressions were detected by Western blot.Re-sults:The expression of CPEB4 was higher in 22 cases of tumor tissue than that in adjacent normal brain tissue.CPEB4 expression was related with pathological grade and KPS score ( P <0.05 ) .When downregulating CPEB4 expression in SKMG-4 cells by siRNA, pierced cells in siCPEB4#2 group were significantly less than those in siControl group ( P <0.001), and the expressions of MMP2 and MMP9 were also reduced.When upregulating CPEB4 expression in T98 cells by pcDNA-3.1 plasmid, pierced cells in control group were significantly more than those in pcDNA-3.1-CPEB4 group( P<0.05), and the expressions of MMP2 and MMP9 were also increased.Conclusion:The expression of CPEB4 is higher in brain glioma tissue.CPEB4 could regulate glioma cell invasion and migration ability by regulating the expression of MMP2 and MMP9.关键词
CPEB4/胶质瘤/侵袭/迁移Key words
CPEB4/glioma/invasion/migration分类
医药卫生引用本文复制引用
刘志军,周海存,陈小兵,刘红林,王贵聪..人脑胶质瘤组织中 CPEB4的表达及与细胞迁移和侵袭的关系[J].郑州大学学报（医学版）,2016,51(5):622-626,5.基金项目
河南省科技发展计划项目162102310162 （）
