郑州大学学报（医学版）2016，Vol.51Issue(5)：643-646,4.DOI:10.13705/j.issn.1671-6825.2016.05.022
河南省商丘市艾滋病抗病毒治疗病毒未抑制患者 HIV-1耐药毒株的变异
Genotype resistant mutations among patients failed to high antiretroviral therapy in Henan Province
摘要
Abstract
Aim:To analyze the prevalence and diversity of genotype resistant mutations among patients failed to high antiretroviral therapy( HAART) in Henan Province.Methods:A total of 231 serum specimens of AIDS individuals with vi-rus load≥1 000 copies/mL were collected and had received more than six months treatment with HAART.The HIV-1 pol gene was amplified by RT-PCR,the sequences were then submitted to the Stanford University HIV drug resistance database to analyze the prevalence of resistant mutations.Results: A total of 229 plasma samples were amplified successfully for protease and reverse transcriptase fragments.Out of 229 sequences,185(80.79%),150(65.50%) and 3(1.31%) se-quences occurred resistance mutations for non-nucleotide reverse transcriptase inhibitors( NNRTIs) ,nucleotide reverse tran-scriptase inhibitors(NRTIs) and protease inhibitors(PIs), respectively.The most common mutations to NNRTIs, NRTIs, and PIs were K103N/S 45.42%(104/229), M184I/V 43.67%(100/229) and M46I 0.87%(2/229), respectively.Un-ivariate logistic regression analysis showed that among AIDS individuals, virus load 5-lg copies/mL decreased the risk of M184I/V mutation(OR=0.283,95%CI=0.136-0.592,P<0.001) compared with virus load 3-lg copies/mL.More than 48 months treatment with HAART had high rate of K103N/S mutation( OR=7.422,95%CI=1.637-33.651,P<0.001), compared with 6 -months treatment.HAART with D4T was associated with high K65R mutation rate(P =0.044).Conclusion:The prevalence of HIV-1 drug resistance with complex patterns is higher among patients failed to HAART in Henan.Selecting more suitable antiretroviral treatment regimen is necessary to reduce the emergence of the drug resistant strains.关键词
人类免疫缺陷病毒/耐药/抗病毒治疗/基因突变/河南省Key words
human immunodeficiency virus/drug resistance/antiretroviral treatment/gene mutation/Henan Province分类
医药卫生引用本文复制引用
袁源,刘宏伟,刘春华,王哲,阮玉华,邢辉..河南省商丘市艾滋病抗病毒治疗病毒未抑制患者 HIV-1耐药毒株的变异[J].郑州大学学报（医学版）,2016,51(5):643-646,4.基金项目
河南省重点科技攻关计划项目102102310003，112102310005；河南省卫生科技创新型人才工程专项经费资助项目2010年 （）
