激光技术2016，Vol.40Issue(5)：638-642,5.DOI:10.7510/jgjs.issn.1001-3806.2016.05.005
基于荧光微球的显微镜点扩散函数修正模型
An optimized model of point spread function of microscopy based on fluorescence beads
摘要
Abstract
In order to eliminate effect of fluorescent bead diameter on measurement results in traditional microscopic point spread function measurement method and improve the measurement accuracy , 2-D equivalent concentration distribution of fluorescent beads was deduced based on theoretical simulation and least square fitting method .The imaging process of fluorescent beads was simulated .The relationships among fluorescent beads diameter , full width at half maximum ( FWHM) of fluorescent beads intensity distribution and FWHM of system point spread function were analyzed by using the least square fitting method and residual error fitting method .Precise FWHM correction model of point spread function was obtained .The results show that the relative error of point spread function FWHM is about 1%when measuring system point spread function with 100nm fluorescent beads.The precise measurement of point spread function is realizable with the optimized model .关键词
显微/点扩散函数/荧光微球/非线性最小二乘拟合Key words
microscopy/point spread function/fluorescent bead/nonlinear least square fitting分类
数理科学引用本文复制引用
鹿伟民,杨西斌,文刚,郑贤良,李辉,熊大曦..基于荧光微球的显微镜点扩散函数修正模型[J].激光技术,2016,40(5):638-642,5.基金项目
国家自然科学基金资助项目（61475185）；国家自然科学基金青年科学基金资助项目（61405238）；中国科学院百人计划资助项目 （）
