辽宁农业科学Issue(4)：23-26,4.DOI:10.3969/j.issn.1002-1728.2016.04.005
转基因抗虫大豆MON87701品系实时荧光PCR检测方法的建立
Establishment of Event Specific Real-time PCR Method for Detection of Genetically Modified Soybean MON8770 1
摘要
Abstract
In order to provide a basis for detection of genetically modi?ed soybean strains MON87701,an e-vent-specific real-time PCR method for the detection of genetically modified soybean MON87701 was estab-lished in this study.Primers and TaqMan probe were designed based on the event-specific sequence of MON87701 soybean.The specificity,sensitivity and repeatability of the developed method were examined,re-spectively.The results indicate this method had good detection specificity and repeatability for genetically modified soybean strains,with its sensitivity reaching 0.01% (m/m).These results showed that the strain-specific real-time PCR method was suitable for the identification of genetically modified soybean MON87701,it can be used to testing genetically modified samples.关键词
转基因大豆/MON87701 品系/实时荧光 PCR/品系鉴定Key words
Genetically modified soybean/MON87701 strains/Real-time PCR/Event specific detection分类
生物科学引用本文复制引用
付海滨,张敏,吴渺渺,韩宏乾,陈丽静,于洋..转基因抗虫大豆MON87701品系实时荧光PCR检测方法的建立[J].辽宁农业科学,2016,(4):23-26,4.基金项目
沈阳市科技计划项目 （）
