陕西医学杂志2016,Vol.45Issue(9):1256-1257,2.DOI:10.3969/j.issn.1000-7377.2016.09.075
志贺菌实验室快速分子检测与分析
Rapid detection and analysis in an outbreak of infectious diarrhea caused by Shigella spp
摘要
Abstract
Objective:To analysis the cause of an outbreak of infectious diarrhea in Binxian County ,Xian‐yang .Methods :Cultured according to the GB4789 .5‐2012 ,meanwhile Seeplex kit and the method of multiplex PCR was used for rapid detection .Results:Nucleic acid showed Shigella virulence gene positive ipaH positive .Conclusion:The outbreak was caused by Shigella spp .Seeplex and the multiplex PCR method compared with the traditional bac‐terial cultured method are more efficient .关键词
腹泻/病因学/腹泻/诊断/志贺菌属/诊断/聚合酶链反应/快速检测Key words
Diarrhea/etiology/Diarrhea/diagnosis/Shigella/diagnosis/Polymerase chain reaction/Rapid detection分类
医药卫生引用本文复制引用
石一,张铮,张义,马国柱,刘长宏,陈飒,刘峰,刘东立..志贺菌实验室快速分子检测与分析[J].陕西医学杂志,2016,45(9):1256-1257,2.基金项目
陕西省卫生和计划生育委员会卫生科研项目 ()