现代医药卫生2016，Vol.32Issue(17)：2650-2651,2655,3.DOI:10.3969/j.issn.1009-5519.2016.17.010
1，25-（OH）2D3对单侧输尿管梗阻模型大鼠Erbin表达的影响
Effect of 1,25-(OH)2D3 on Erbin expression in unilateral ureteral obstruction rat model
张国锐 1林智峰 1杨晓萍 1赵瑾 2陶林2
作者信息
- 1. 石河子大学医学院 第一附属医院肾病科，新疆石河子832008
- 折叠
摘要
Abstract
Objective To investigate the effect of activated vitamin D3-1,25-(OH)2D3 on the Erbin expression in uni lateral ureteral obstruction(UUO)rat. Methods Ninety-six SD rats were randomly divided into the normal control group,interven-tion group,sham operation group and UUO model group according to the random number table method ,24 cases in each group. The UUO model in the intervention group and the UUO model group was established by freeing and ligating the left ureter at the inferior polar of the left kidney,the left ureter in the sham operation group was only dissociated after lapatotomy,while the inter-vention group was given activated vitamin D3 0.5μg and peanut oil1 mL by gavage from the beginning of model construction ,once daily,for 14 d. Six rates in each group were killed on postoperative 1,3,7,14 d. The immunohistochemical method was used to detect the expression of TGF-β1 and Erbin in each group. Results The expressions of Erbin and TGF-β1 in the UUO model group were significantly higher than those in the normal control group and sham operation group ,the differences were statistically significant(P<0.05);the expression of Erbin in the intervention group was significantly higher than that in the model group ,while the TGF-β1 expression was significantly lower than that in the model group,the difference was statistically significant(P<0.01). Conclusion 1,25-(OH)2D3 may increase the Erbin expression in renal tissue of UUO rat,inhibits the TGF-β1 expression and alleviates renal interstitial fibrosis.关键词
维生素D/肾小球硬化症,局灶节段性/转化生长因子β1/ErbinKey words
Vitamin D/Glomerulosclerosis,focal segmental/Transforming growth factor beta1/Erbin引用本文复制引用
张国锐,林智峰,杨晓萍,赵瑾,陶林..1，25-（OH）2D3对单侧输尿管梗阻模型大鼠Erbin表达的影响[J].现代医药卫生,2016,32(17):2650-2651,2655,3.
