反相高效液相色谱法同时测定丹参中5种成分含量OACSTPCD
A Simultaneous Determination of Five Components in Radix et Rhizoma Salviae Miltiorrhizae by RP-HPLC
目的:建立高效液相色谱法同时测定丹参中3种水溶性和2种脂溶性成分。方法采用反相高效液相色谱法,Welchrom® C18(250 mm×4.6 mm,5μm)色谱柱,以乙腈(A)-0.1%磷酸(B)为流动相梯度洗脱:0~15 min,10%→12%A;~35 min,12%→20%A;~45 min,20%→60%A;~65 min,60%→65%A;~80 min,65%→80%A;~90 min,10%A。流速1 mL•min-1,检测波长2…查看全部>>
Objective To develop a method for simultaneous determination of three hydrophilic components and two lipophilic components in Radix et Rhizoma Salviae Miltiorrhizae. Methods The RP-HPLC method was performed by using a Welchrom C18 column(250 mm×4.6 mm,5 μm)with a mobile phase of acetonitrile (A)-0.1%phosphoric acid(B). The gradient elution program was as follows:0-15 min,10%→12%A;-35 min,12%→20%A;-45 min,20%→60%A;-65 min,60%→65%A;-80 min,65%→80%A;-90 m…查看全部>>
姜晓燕;张琳;窦志华;陈霞;王征宇
江苏省南通市中医院药剂科,南通 226001江苏省南通市第三人民医院药剂科,南通 226006江苏省南通市第三人民医院药剂科,南通 226006江苏省南通市第三人民医院药剂科,南通 226006江苏省南通市第三人民医院药剂科,南通 226006
医药卫生
丹参色谱法,反相高效液相丹参素迷迭香酸丹酚酸 B隐丹参酮丹参酮ⅡA含量测定
Radix et Rhizoma SalviaemiltiorrhizaeChromatogrophy, reversed phase-high performance liquidTanshinolRosmarinic acidSalviamolic acid BClyptotanshinonTanshinone II AContent determination
《医药导报》 2016 (9)
997-1000,4
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