中国药房2016，Vol.27Issue(24)：3440-3442,3.DOI:10.6039/j.issn.1001-0408.2016.24.40
传统煎法与现代煎药机制备四逆汤的HPLC指纹图谱对比研究
Comparative Study on the HPLC Fingerprint of Sini Decoction Prepared by Traditional Decoction and Mod-ern Machine Decoction
黄育生 1唐洪梅 2招远明 2王婷 1柴玉娜 1刘媛 1钟如帆1
作者信息
- 1. 广州中医药大学第一临床医学院，广州510006
- 2. 广州中医药大学第一附属医院，广州 510405
- 折叠
摘要
Abstract
OBJECTIVE:To establish the HPLC fingerprints for Sini decoction and compare the differences of compositions of Sini decoction prepared by traditional decoction and modern machine decoction. METHODS:HPLC was performed on the column of Kromasil C18 with mobile phase of acetonitrile-0.1% phosphoric acid(gradient elution)at a flow rate of 1.0 ml/min,the detec-tion wavelength was 235 nm,column temperature was 30 ℃,and injection volume was 10 μl. The HPLC fingerprints of 10 batch-es of Sini decoction were determined with reference peak of liquiritin peaks,and common peak identification and similarity evalua-tion were conducted by using Chromatographic Fingerprint Similarity Evaluation System(2004 A edition). RESULTS:There were 18 common peaks and the similarity was no less than 0.982. According to the verification,the fingerprint of 10 batches of Sini de-coction showed good similarity with reference fingerprint,and the similarity of 10 batches of Sini decoction was high,which was prepared by the 2 methods. CONCLUSIONS:The established fingerprint is specific and stable,and can provide reference for quali-ty evaluation and control for Sini decoction;and there are no obvious differences in the main chemical compositions of Sini decoc-tion prepared by traditional decoction and modern machine decoction.关键词
传统煎法/煎药机/四逆汤/指纹图谱Key words
Traditional decoction/Decoction machine/Sini decoction/Fingerprint分类
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黄育生,唐洪梅,招远明,王婷,柴玉娜,刘媛,钟如帆..传统煎法与现代煎药机制备四逆汤的HPLC指纹图谱对比研究[J].中国药房,2016,27(24):3440-3442,3.
