中国药理学通报2016，Vol.32Issue(9)：1208-1216,1217,10.DOI:10.3969/j.issn.1001-1978.2016.09.006
DSF-Cu通过影响线粒体功能和细胞骨架诱导鼻咽癌CNE-2 Z细胞凋亡
DSF-Cu induces apoptosis of human nasopharyngeal carcinoma CNE-2 Z cells by affecting function of mitochondria and cytoskeleton
摘要
Abstract
Aim To study the mechanism of DSF-Cu induced apoptosis of human nasopharyngeal carcinoma CNE-2 Z cells by affecting the function of mitochondria and cytoskeleton. Methods The cell cycle,the rate of apotosis,the levels of intracellular ROS and MMP in CNE-2 Z cells were tested by flow cytometry after trea-ted with different concentration of DSF-Cu. The chan-ges of the cell surface morphology, ultrastructure, cell height, width and roughness were detected by AFM. The distribution and reorganization of cytoskeleton F-actin were observed by Laser scanning confocal micro-scope. Results Cells were incubated with different concentration of DSF-Cu ( 0 ~200 nmol · L-1 ) for 24 h, the apoptotic ratio increased significantly and the treatment of DSF-Cu resulted in a concentration-de-pendent accumulation of CNE-2Z cells in G2/M phase. Furthermore,the treatment of DSF-Cu was able to in-crease the production of intracellular ROS and decrease the MMP in CNE-2Z cells. In addition,AFM imaging showed that compared to the control group,with the in-crease of DSF-Cu concentration,the CNE-2Z cells be-came smaller, cytoplasm condensed, the height in-creased,and the surface roughness reduced. Moreover, the filopodia became shorter, shrinked and even com-pletely destroyed after treated with different concentra-tion of DSF-Cu. At last,the LSCM image showed that the fluorescence intensity of F-actin networks was de-creased, then the structure was rearranged and de-stroyed obviously by treated with DSF-Cu. Conclusion DSF-Cu can induce apoptosis and arrest cell cycle at G2/M phase in CNE-2Z cell through a mitochondria-dependent pathway. Above findings highlight the appli-cations of AFM at the single cell level for the investiga-tion of antineoplastic drug in nasopharyngeal carcinoma therapy.关键词
双硫仑/原子力显微镜/细胞骨架/活性氧/凋亡/鼻咽癌/线粒体膜电位Key words
disulfiram/atomic force microscope/cy-toskeleton/ROS/cell apoptosis/nasopharyngeal carci-noma/mitochondrial membrane potential分类
医药卫生引用本文复制引用
杨亚萍,朱林燕,汪亚帷,李梦佳,张可凡,孙晓雪,梁志红,陈丽新,王立伟,杨海峰..DSF-Cu通过影响线粒体功能和细胞骨架诱导鼻咽癌CNE-2 Z细胞凋亡[J].中国药理学通报,2016,32(9):1208-1216,1217,10.基金项目
国家自然科学基金资助项目(No 31070997,81372382) （No 31070997,81372382）
广东省自然科学基金资助项目( No S2013010013780,2015A 030313363) （ No S2013010013780,2015A 030313363）
广东省医学科研基金项目(No A201506) （No A201506）
