中国药理学通报2016，Vol.32Issue(9)：1217-1223,7.DOI:10.3969/j.issn.1001-1978.2016.09.007
人参皂苷Rc、Re、Rf 和Rg1对药物代谢酶CYP1 A1活性诱导作用研究
Study on the induction of drug metabolizing enzyme CYP1 A1 activity by Ginsenoside Rc,Re,Rf and Rg1
摘要
Abstract
Aim To investigate the induction effect of ginsenoside Rc, Re, Rf and Rg1 on CYP1A1, and further validate the role of aryl hydrocarbon receptor in CYP1A1 expression. Methods Dual luciferase re-porter gene system was performed. Four kinds of gin-senoside were screened for aryl hydrocarbon receptor activation by reporter assays, and TCDD as the positive control. Further with different concentrations of ginsen-oside Rc, Re, Rf and Rg1 treated on LS174T cells, RNA and total protein were extracted to detect the reg-ulating effect of ginsenosides on CYP1 A1 mRNA and protein expression with Real-time PCR and Western blot technology respectively. Results Reporter gene screening showed that the ginsenoside Rc, Re, Rf and Rg1 could activate AhR and had potential effects on the induction of CYP1A1 enzyme. Meanwhile, dose-de-pendent induction of the gene expression were observed in response to ginsenoside Rc, Re, Rf and Rg1 and the levels of CYP1 A1 protein expression were increased by ginsenoside Rc, Re, Rf and Rg1 in varying de-grees. Conclusion Ginsenoside Rc, Re, Rf and Rg1 can up-regulate the gene and protein expression of CYP1 A1 possibly via the AhR-mediated CYP1 A1 path-way.关键词
人参皂苷/AhR/CYP1A1/报告基因/实时荧光定量PCR/Western blot/药物相互作用Key words
ginsenoside/AhR/CYP1 A1 reporter gene/Real-time PCR/Western blot/drug interactions分类
医药卫生引用本文复制引用
李晗,王宇光,马增春,谭洪玲,肖成荣,汤响林,张伯礼,高月..人参皂苷Rc、Re、Rf 和Rg1对药物代谢酶CYP1 A1活性诱导作用研究[J].中国药理学通报,2016,32(9):1217-1223,7.基金项目
“十二五”国家科技重大专项资助项目( No 2014ZX09304307001-003,2015ZX09501004003-003) （ No 2014ZX09304307001-003,2015ZX09501004003-003）
