中国药理学通报2016，Vol.32Issue(9)：1249-1252,1253,5.DOI:10.3969/j.issn.1001-1978.2016.09.012
多吡啶钌配合物[（Phen）2 Ru（dppz）]（PF6）2的抗菌活性及机制研究
Analysis of antibacterial activity and mechanism of polypyridyl ruthenium complex
摘要
Abstract
Aim To analyze the antibiotic activity and mechanism of a polypyridyl ruthenium complex. Meth-ods The antibacterial activity of [ ( Phen ) 2 Ru ( dp-pz) ] ( PF6 ) 2 was determined by MIC and MBC value. Based on a fluorescent activity of this complex, the flu-orescent emission spectra was used to analyze the com-bination of complex to DNA. Then the competition combination was analyzed between complex and Gold View to DNA. Lastly, gel electrophoresis of DNA was applied to detect the combination situation between complex and DNA. Results This kind of polypyridyl ruthenium complex showed a significant antibacterial activity with a minimum antibacterial conentration of 0. 2~0. 4 g · L-1 . That was caused by the combina-tion and distortion of DNA due to the activity of this complex. Conclusion The antibacterial activity and the mechanism of antibacterial activity about [ ( Phen) 2 Ru( dppz) ] ( PF6 ) 2 are confirmed in this re-search, which provides a good foundation for the devel-opment of such class of compound.关键词
多吡啶钌配合物/抑菌活性/荧光检测/代谢活性/DNA结合/DNA降解Key words
polypyridyl ruthenium complex/antibacte-rial activity/fluorescent analysis/metabolism/DNA combination/DNA degradation分类
医药卫生引用本文复制引用
刘汉杰,付彬,付爱玲,付琛..多吡啶钌配合物[（Phen）2 Ru（dppz）]（PF6）2的抗菌活性及机制研究[J].中国药理学通报,2016,32(9):1249-1252,1253,5.基金项目
国家自然科学基金资助项目( No 81273416) （ No 81273416）
教育部高校基本科研业务费(No XDJK2013A030) （No XDJK2013A030）
教育部回国人员启动基金(No 2012-940) （No 2012-940）
教育部新世纪优秀人才支持计划资助 （）
