中国药理学通报2016，Vol.32Issue(9)：1301-1305,1306,6.DOI:10.3969/j.issn.1001-1978.2016.09.022
绞股蓝皂苷对AGEs诱导下人肾小球系膜细胞中RAGE及转化生长因子-β1表达的影响
Effect of gypenosides on RAGE and TGF-β1 expression in human mesangial cells induced by AGEs
摘要
Abstract
Aim To observe the effect of gypenosides ( GP) on the expression of receptor for advanced gly-cated endproducts ( RAGE ) and transforming growth factor-β1 ( TGF-β1 ) in human mesangial cells( HMCs) induced by AGEs. Methods HMCs were cultured in DMEM of low glucose containing 15% fetal bovine ser-um in vitro, which were divided into four groups: the normal group, model group, GP group and positive control group. In addition to the normal group, the other groups were stimulated by AGEs ( 200 mg · L-1 );furthermore, GP group was intervened with dif-ferent concentrations(25,75,175 mg·L-1) of GP, while control group was given 10 mmol · L-1 of amin-oguanidine hydrochloride. The expression of RAGE and TGF-β1 protein of each group was detected by Western blot; the expression of RAGE and TGF-β1 mRNA of each group was detected by RT-PCR. Re-sults The expression of RAGE, TGF-β1 protein and mRNA in HMCs induced by AGEs in the model group was significantly higher than that of the normal group ( P<0. 01 );compared with the positive control group ( P<0. 01 ) , GP could obviously reduce the expression of RAGE, TGF-β1 protein and mRNA in a dose-de-pendent manner. Conclusion GP can reduce the ex-pression of RAGE in HMCs induced by AGEs, block AGEs-RAGE signaling pathway and decrease the ex-pression of the downstream factor TGF-β1 , therefore, it plays the role in the resistance of rennal fibrosis in DN.关键词
绞股蓝皂苷/糖尿病肾病/人肾小球系膜细胞/晚期糖基化终末产物/晚期糖基化终末产物受体/转化生长因子-β1/纤维化Key words
gypenosides/diabetic nephropathy/human mesangial cells/advanced glycation endproducts/recep-tor for advanced glycated endproducts/TGF-β1/fibrosis分类
医药卫生引用本文复制引用
张秋艳,唐灵,王艳,周康,张慧云..绞股蓝皂苷对AGEs诱导下人肾小球系膜细胞中RAGE及转化生长因子-β1表达的影响[J].中国药理学通报,2016,32(9):1301-1305,1306,6.基金项目
广西自然科学基金资助项目(No 2013GXNSFAA019197) （No 2013GXNSFAA019197）
广西壮族自治区卫计委项目( No Z2016387) （ No Z2016387）
桂林市科学研究与技术开发计划项目(No 20120121-1-1) （No 20120121-1-1）
