作物研究2016，Vol.30Issue(5)：481-486,511,7.DOI:10.16848/j.cnki.issn.1001-5280.2016.05.01
利用SSR标记构建水稻核心亲本指纹图谱
Fingerprinting Construction of Rice Core Parental Lines with SSR Markers
摘要
Abstract
Fingerprinting profiles and molecular identity cards of 27 rice core parental lines were established using 48 SSR markers recommended by “Sector Standard of Agriculture (NY/T 1433 -2014)”.Total 162 Polymorphic fragments were detected with an average of 3.53 alleles for each SSR primer.Each of the 45 SSR primers are amplified more than 2 alleles respectively,their PIC (polymeric index content) values ranged from 0.07 to 0.80,and the average PIC value is 0.46.Result from cluster analysis showed that genetics similarities among the 27 lines ranged from 0.593 to 0.944,which reflected basically the genetic relatives based on the pedigree analysis.Varietal genetic files were developed based on the established fingerprinting profiles and molecular identity cards,and found that it performed well in intellectual property rights protection,varietal identification and purity testing.关键词
水稻/核心亲本/SSR标记/指纹图谱/分子身份证Key words
rice/core parental lines/SSR markers/fingerprinting/molecular identity cards分类
农业科技引用本文复制引用
曾晓珊,彭丹,石媛媛,谢伟,刘爱民..利用SSR标记构建水稻核心亲本指纹图谱[J].作物研究,2016,30(5):481-486,511,7.基金项目
公益性行业（农业）科技专项（201303005）。 （）
