作物研究2016，Vol.30Issue(5)：487-491,5.DOI:10.16848/j.cnki.issn.1001-5280.2016.05.02
分子标记辅助选择 Pi9基因改良 R288的稻瘟病抗性
I mproving Blast Resistance of Rice Restorer R288 by Molecular Marker -Assisted Selection of Pi9 Gene
摘要
Abstract
To improve blast resistance of rice restorer line R288,an inner -gene functional marker Clon2 -1 was designed based on DNA sequence of the cloned broad -spectrum blast resistance gene Pi9,and molecular marker -assisted breed-ing was implemented.The main results were as the follows:Clon2 -1 is a co -dominant marker and showed obviously and stable polymorphism between Pi9 gene donor parent 75 -1 -127 and the receptor R288,and selection efficiency of Clon2-1 marker genotype for rice blast resistance phenotype reached 100%.The BC6 F3 population harboring Pi9 with R288 background was developed through consecutive backcross and self -pollination,and thereupon a resistant homozygous line R288 -Pi9 was bred.The hybrid produced by R288 -Pi9 and the photo -thermal sensitive genic male sterile line Peiai 64S,also showed high -level blast resistance.关键词
水稻/稻瘟病抗性/Pi9基因/分子标记辅助选择育种Key words
rice/blast resistance/Pi9 gene/molecular marker -assisted selection breeding分类
农业科技引用本文复制引用
行璇,王国梁,刘雄伦,陈海龙,杨丰宇,李永聪,廖花,游亮,刘金灵,戴良英..分子标记辅助选择 Pi9基因改良 R288的稻瘟病抗性[J].作物研究,2016,30(5):487-491,5.基金项目
国家自然科学基金（3l171526）；国家转基因生物新品种培育重大专项（2015ZX08001-002）；湖南省重大专项（2015NK1001）；教育部高校创新团队发展计划（IRT1239）。 （）
