重庆医学2016，Vol.45Issue(27)：3748-3752,5.DOI:10.3969/j.issn.1671-8348.2016.27.002
TCF21基因对肺腺癌 A549细胞DDP化疗及放疗敏感性的影响
The effects of TCF21 genes on radiation and chemotherapy sensitivity of A549 lung adenocarcinoma cells
摘要
Abstract
Objective To investigate the transcription factor 21 (transcription factor 21 ,TCF21) gene on human lung cancer A549 cells sensitivity to chemotherapy .Methods Using lentivirus technology in A549 lung cancer cells highly expressed genes TCF21 ,fluorescence quantitative PCR ,Western Blot analysis were used to analyse the expression of the target gene ,MTT assay was used to detect the effect of TCF21 lung adenocarcinoma A549 cells on cisplatin (cis‐Dichlorodiamineplatinum ,DDP) chemosen‐sitivity ,and colony assay was used to detect the effect of overexpression of TCF21 lung adenocarcinoma A549 cells on radiosensitiv‐ity .Results After 72 h ,with the increasing concentration of DDP (0 ,0 .625 ,1 .250 ,2 .500 ,5 .000 ,10 .000 mg/L) ,corresponding in‐hibition rates in each group increased ,and the inhibition rate of the high expression group was significantly higher in empty vector group and untransfected group (P<0 .05) ,no significant difference between the two then;overexpression TCF21 group with drug concentration and time and increase the rate of high expression inhibition corresponding increase (P<0 .05) ;after receiving X radi‐ation ,non‐transfected group ,untransfected plus radiotherapy group ,vector group ,vector plus radiotherapy group ,high expression and high expression + radiotherapy colony formation rates were:95 .17% ± 2 .85% ,88 .20% ± 2 .03% ,93 .80% ± 4 .17% , 85 .60% ± 2 .42% ,71 .67% ± 3 .21% ,56 .00% ± 2 .65% .Conclusion TCF21 gene expression can significantly enhance the sensitiv‐ity to radiotherapy and chemotherapy DDP A549 lung cancer cells .关键词
肺肿瘤/放射疗法/药物疗法/A549/TCF21/敏感性Key words
lung neoplasms/radiotherapy/chemotherapy/A549/TCF21/sensitivity分类
医药卫生引用本文复制引用
陆晓,冼磊..TCF21基因对肺腺癌 A549细胞DDP化疗及放疗敏感性的影响[J].重庆医学,2016,45(27):3748-3752,5.基金项目
广西自然科学基金资助项目（2013GXNSFAA019270）。 （）
