重庆医学2016，Vol.45Issue(27)：3757-3759,3762,4.DOI:10.3969/j.issn.1671-8348.2016.27.004
二膦酸盐对人骨肉瘤细胞诱导凋亡作用的实验研究
Apoptotic effects of bisphosphonate inosteosarcoma MG-63 cells in vitro
摘要
Abstract
Objective To observe the effects of bisphosphonate on the inhibit proliferation and the apoptosis effect in osteo‐sarcoma MG‐63 cells in vitro ,explore the phosphonic acid salt of bone sarcoma cells ,induce apoptosis and its possible mechanism . Methods Sixty three osteosarcoma MG‐63 cells were cultured in vitro .After treated with bisphosphonate 400 μg/mL ,without bi‐sphosphonate but normal saline ,they were incubated 72 h after the application of the two group cell immunofluorescence test ;then observe the expression of apoptosis factors Caspase 3 and Fas ;Flow cytometry detection line was used to detect the osteosarcoma cell line MG‐63 cells apoptosis rate of each group .Results 72 h after treatment with bisphosphonate ,the expression of apoptosis factor of Caspase‐3 and Fas in osteosarcoma MG‐63 cells were strongly expressed ,and it was observed by immunofluorescent assay , while in blank control group ,we could barely see the expression of apoptosis factors Caspase‐3 and Fas ;Flow cytometry test results showed that two phosphonic acid salt 400 μg/mL intervention group cell apoptosis rate was 54 .00% ,far more than normal saline blank control group ,of which the apoptosis rate was 3 .10% ,the difference was statistically significant (P<0 .05) ,there is an obvi‐ous phenomenon of induced apoptosis .Conclusion Bisphosphonate has a strong apoptotic effects of bisphosphonate in osteosarcoma MG‐63 cells in vitro .Bisphosphonate can inhibit osteolysis of osteosarcoma MG‐63 cells via regulating the expression of Caspase‐3 , Fas in osteosarcoma MG‐63 cells .Bisphosphonate may serve as a potential therapeutic agent for treatment of osteosarcoma .关键词
二膦酸盐类/骨肉瘤/细胞凋亡/MG-63细胞株Key words
bisphosphonates/osteosarcoma/apoptosis/M G-63 cells分类
医药卫生引用本文复制引用
买买提艾力·哈斯木,江仁兵,白靖平,徐万龙..二膦酸盐对人骨肉瘤细胞诱导凋亡作用的实验研究[J].重庆医学,2016,45(27):3757-3759,3762,4.基金项目
国家自然科学基金资助项目（81360276）；新疆维吾尔自治区卫生厅青年科技人才专项科研资金（2012Y07）；新疆医科大学创新基金（XJC201157）。 （）
