重庆医学2016，Vol.45Issue(27)：3766-3769,4.DOI:10.3969/j.issn.1671-8348.2016.27.007
基于凝血酶合并缺氧诱导PC12细胞损伤的模型研究
Model of PC12 cell injury induced by thrombin combined with hypoxia
摘要
Abstract
Objective To explore the optimal reaction time of thrombin combined with hypoxia on PC12 cell injury ,and to provide experimental method for the study of the significance of thrombin in ischemic brain injury .Methods the PC12 cell injury model induced by hypoxia in vitro was established .In the conventional cell culture method ,three 1% O2 (5% CO2 ,6 h ,94% N2 ) and DMEM (24 h) cells were used to simulate ischemia ,and four (50 ,150 and 200 U/ml) were added to the different concentrations of thrombin (0 ,100 ,and 12 h) .Cell survival rate was detected by MTT and apoptosis rate of cell was detected by TUNEL . Results the survival rate of the cells decreased ,the positive rate and apoptosis rate of TUNEL cells increased gradually with the in‐crease of time and the concentration of thrombin .Low concentrations of thrombin (50 U/mL) in hypoxia 1H ,compared with the control group (0 U/mL) ,the survival rate increased ,suggesting that low thrombin may have protective effect .The cell viability was significantly decreased (P<0 .01) ,and the apoptosis rate was significantly increased (P<0 .05) ,and the difference was statistically significant( P< 0 .05 ) between 200 U/mL and control group (U/mL ) after 12 h was significantly decreased (12 h ) . Conclusion Thrombin combined with hypoxia in different time can cause cell damage ,with the increase of time and thrombin con‐centration ,the damage of cells increased gradually ,the MTT and TUNEL cells apoptosis ,the minimum dose of thrombin induced damage to PC12 cells ,150 U/mL thrombin in the role of 12 h cells after the damage and compared with the control group ,it has sig‐nificant statistical significance ,therefore ,the optimal model for the damage of PC12 cells after 150 U/mL was selected .关键词
PC12细胞/凝血酶/缺氧/细胞损伤Key words
PC12 cells/thrombin/anoxia/cell injury分类
医药卫生引用本文复制引用
覃弘宇,李银,李定祥,邓奕辉..基于凝血酶合并缺氧诱导PC12细胞损伤的模型研究[J].重庆医学,2016,45(27):3766-3769,4.基金项目
湖南省自然科学基金资助项目（13JJ6061，14JJ2113）；湖南省中医药科研基金资助项目（201319）；湖南省教育厅资助项目（12A105）；湖南省重点学科中西医结合基础资助项目；湖南省自然科学创新群体基金资助项目；湖南省高校科技创新团队资助。 （）
