重庆医学2016,Vol.45Issue(27):3777-3779,3782,4.DOI:10.3969/j.issn.1671-8348.2016.27.010
食管癌变进程中间质成纤维细胞的表型变化
Change of phenotype of stromal fibroblasts in esophageal carcinogenesis
摘要
Abstract
Objective To investigate the expressions ofα‐SMA in different esophageal stromal fibroblasts in esophageal car‐cinogenesis .Methods IHC method was uesd to detect the expression of α‐SMA protein in stromal fibroblasts of twenty normal e‐sophageal tissues ,eighty precancerous lesions and fifty esophageal carcinomas respectively .Three kinds of esophageal stromal fibro‐blasts were cultured primarily and cells immunohistochemical staining was carrired out after being purified .Expression of α‐SMA was detected by RT PCR .Results IHC results showed thatα‐SMA expressions in normal ,precancerous and cancerous lesions were of significant differences .RT‐PCR results showed thatα‐SMA expressions were different significantly among three kinds of fibro‐blasts .Conclusion Esophageal stromal fibroblasts were activated with carcinogenensis .AFs was possibly the origion of CAFs .关键词
食管肿瘤/间质细胞/间质成纤维细胞/癌变/表型Key words
esophageal neoplasms/stromal cells/stromal fibroblast/carcinogenesis/phenotype分类
医药卫生引用本文复制引用
徐志彬,袁丽,郑秀丽,王士杰,吴明利..食管癌变进程中间质成纤维细胞的表型变化[J].重庆医学,2016,45(27):3777-3779,3782,4.基金项目
河北省卫生厅医学科学研究重点课题计划(20160656)。 ()