动物营养学报2016，Vol.28Issue(9)：2715-2723,9.DOI:10.3969/j.issn.1006-267x.2016.09.008
饲粮中添加苜蓿草粉对生长猪结肠微生物区系及其代谢产物的影响
Effects of Dietary Alfalfa Meal Supplementation on Colonic Microbiota and Its Metabolites in Growing Pigs
摘要
Abstract
The present study aimed to investigate the effects of insoluble dietary fiber from alfalfa meal on co-lonic microbiota and its fermentation metabolites in growing pigs. In this experiment, 24 castrated male pigs ( Duroc×Landrace×Yorkshire) with initial body weight of (24.8±0.7) kg were randomly divided into 4 groups with 6 replicates per group and 1 pig per replicate. Pigs in each group were fed one of four diets (0, 5%, 10%and 15% levels of alfalfa meal) . Pigs were slaughtered after 28 d for sampling. Then we determined serum fat-ty acid composition, short-chain fatty acid ( SCFA) concentration and microbiota composition in colonic diges-ta. The results showed that diets with alfalfa meal significantly decreased feed to gain ratio ( P<0.05) , but had no significant effects on average daily gain and average daily feed intake of growing pigs ( P>0.05) . Analysis of colonic microbiota 16S rDNA V3 region revealed that alfalfa meal had no significant effect on microbiota composition ( P>0.05) , but significantly increased total SCFA, acetate and butyrate concentrations in colonic digesta of pigs ( P<0.05) . With the addition of alfalfa meal in diets, polyunsaturated fatty acid content was sig-nificantly increased ( P<0.05) , while the contents of saturated fatty acid and monounsaturated fatty acid were significantly decreased in the serum (P<0.05). The results indicate that insoluble dietary fiber from alfalfa meal has no effect on colonic microbiota structure, but enhances fermentation activity of colonic microbiota and SCFA producing, and also plays a role in regulating fatty acid profile of growing pigs.关键词
生长猪/苜蓿草粉/不可溶性纤维/短链脂肪酸/结肠微生物区系/脂肪酸Key words
growing pigs/alfalfa meal/insoluble dietary fiber/SCFA/colonic microbiota/fatty acid分类
农业科技引用本文复制引用
王嘉为,张蕾,祝皎月,王宇波,尹靖东,朱伟云..饲粮中添加苜蓿草粉对生长猪结肠微生物区系及其代谢产物的影响[J].动物营养学报,2016,28(9):2715-2723,9.基金项目
国家973资金资助项目(2013CB127302,2012CB124702) （2013CB127302,2012CB124702）
北京市级大学生创新训练项目(2015BJ049) （2015BJ049）
