动物营养学报2016，Vol.28Issue(9)：2749-2754,6.DOI:10.3969/j.issn.1006-267x.2016.09.012
妊娠后期胎儿宫内生长受限对蒙古绵羊胎儿肝脏细胞外基质合成的影响
Effects of Fetal Intrauterine Growth Retardation during Late Pregnancy on Extracellular Matrix Synthesis in Fetal Liver of Mongolia Sheep
摘要
Abstract
This study investigated the effects of fetal intrauterine growth retardation ( IUGR) during late preg-nancy on extracellular matrix synthesis in fetal liver of Mongolia sheep. Eighteen healthy Mongolia sheep with synchronization of estrus and conception were allocated to three groups with 6 replicates per group and 1 sheep per replicate. Metabolizable energy ( ME ) level was 0. 175 ( R1 group ) , 0. 330 ( R2 group ) and 0.670 MJ/(kg BW0.75·d) (Ad libitum group, C group), respectively. At day 140 of gestation, ewes were slaughtered to determine parenchyma cell number and extracellular matrix contents in liver of their fetuses. The results showed that liver weight (P<0.01), liver cell number (P<0.05), liver cell nucleus diameter (P<0.01), and the contents of collagen type Ⅰ(P<0.01), collagen type Ⅲ(P<0.05), collagen type Ⅳ (P<0.01), laminin (P<0.01), hyaluronic acid (P<0.05) and fibronectin (P<0.01) in fetal liver of R1 group were significantly lower than those of C group, and endothelial cell number in fetal liver was significantly high-er than that of C group(P<0.05); significantly higher fetal liver weight (P<0.05) and fibronectin content ( P<0.01) were founded in R2 group compared to C group, and endothelial cell number in fetal liver was sig-nificantly enhanced in R1 group compared to C group ( P<0.05) . The results indicate that nutrition restriction during late pregnancy of Mongolia sheep restricts growth and development of fetal liver, and a serious impact on the synthesis of extracellular matrix is found when ME level is 0.175 MJ/(kg BW0.75·d), and liver fibro-sis repair reaction also occurs; only the change of fibronectin content in liver fetal is found when ME level is 0.330 MJ/(kg BW0.75·d).关键词
营养限饲/胎儿肝脏/肝内实质细胞/细胞外基质Key words
nutrition restriction/fetal liver/liver parenchyma cell/extracellular matrix分类
农业科技引用本文复制引用
何珊,刘迎春,李玲瑶,梁玮,高峰..妊娠后期胎儿宫内生长受限对蒙古绵羊胎儿肝脏细胞外基质合成的影响[J].动物营养学报,2016,28(9):2749-2754,6.基金项目
国家自然科学基金(31260559,31460612) （31260559,31460612）
内蒙古自然科学基金杰出青年项目(2013JQ02) （2013JQ02）
内蒙古自治区青年创新人才项目---“草原英才”工程后备人才选拔计划 （）
