动物营养学报2016，Vol.28Issue(9)：2778-2785,8.DOI:10.3969/j.issn.1006-267x.2016.09.016
共轭亚油酸对 C2 C12肌细胞生脂和生肌分化的影响
Effects of Conjugated Linoleic Acid on Adipogenic and Myogenic Differentiation of C2C12 Myoblasts
摘要
Abstract
This study was conducted to evaluated the effects of conjugated linoleic acid ( CLA) on adipogenic trans-differentiation and myogenic differentiation of C2C12 myoblasts. Either c9, t11-CLA or t10, c12-CLA ( the final concentration was 50μmol/L) was used to treat the C2C12 mouse origin myoblasts which were cul-tured and induced adipogenic trans-differentiation and normal myogenic differentiation, respectively. And set the control group. The C2C12 myoblasts adipogenic trans-differentiation on the tenth day and myogenic differ-entiation on the eighth day were used for real-time quantitative PCR detection, and to observed the effects of c9,t11-CLA and t10, c12-CLA on different differentiation of C2C12 myoblasts. The results showed as fol-lows:1) compared with the control group, c9,t11-CLA promoted adipogenic trans-differentiation of C2C12 myoblasts, significantly increased the intracellular triglycerides ( TG ) content and up-regulated intracellular genes expression levels of the fatty acid synthetase ( FAS) , CCAAT/enhancer binding protein α ( C/EBPa) , peroxisome proliferators-activated receptorsγ( PPARγ) and fatty acid binding protein 4 ( FABP4) ( P<0.05) . Compared with the control group, t10,c12-CLA inhibited the adipogenic trans-differentiation of C2C12 myo-blasts, significantly decreased the intracellular TG content and down-regulated intracellular genes expression levels of C/EBPa, PPARγand FABP4 ( P<0.05) . The results of Western blotting showed that protein expres-sion levels of FAS and FABP4 also changed as same as the gene expression levels. 2) Compared with the con-trol group, t10,c12-CLA inhibited the myogenic differentiation of C2C12 myoblasts, significantly decreased the intracellular myotube numbers/cell numbers and down-regulated intracellular genes expression levels of myogenin (MYOG) and myogenic differentiation antigen (MYOD) (P<0.05). Compared with the control group, c9,t11-CLA significantly down-regulated gene expression level of MYOG ( P<0.05) and promoted the myogenic differentiation of C2C12 myoblasts to some degree. The results of Western blotting showed that pro-tein expression levels of MYOG and MYOD also changed as same as the gene expression levels. All these re-sults suggest that CLA has important regulation on normal myogenic differentiation and adipogenesis trans-dif-ferentiation of skeletal muscle cells in animals.关键词
共轭亚油酸/C2C12肌细胞/生脂/生肌/分化Key words
conjugated linoleic acid/C2C12 myoblasts/adipogenic/myogenic/differentiation分类
农业科技引用本文复制引用
齐仁立,王琪,王敬,杨飞云,刘作华,黄金秀..共轭亚油酸对 C2 C12肌细胞生脂和生肌分化的影响[J].动物营养学报,2016,28(9):2778-2785,8.基金项目
国家“973”计划项目(2012CB124702) （2012CB124702）
国家自然科学基金(31302055) （31302055）
重庆市畜牧科学院基本科研业务(14441) （14441）
