动物营养学报2016，Vol.28Issue(9)：2802-2811,10.DOI:10.3969/j.issn.1006-267x.2016.09.019
葡萄原花青素对感染柔嫩艾美尔球虫肉仔鸡生长性能和免疫功能的影响
Effects of Grape Procyanidins on Growth Performance and Immune Function of Broiler Chicks Challenged with Eimeria tenella
摘要
Abstract
This study was conducted to investigate the effects of dietary grape procyanidins ( GPC) on the per-formance and immune function of broiler chicks challenged with Eimeria tenella. A total of 350 one-day-old healthy male broiler chicks ( Cobb-500) were randomly allotted into 5 groups with 7 replicates per group and 10 chicks per replicate. Chicks in blank control ( BC) group and negative control ( NC) group were fed the basal diet, and the others in experimental groups were fed the basal diet supplemented with 7. 5, 15. 0 and 30.0 mg/kg GPC, respectively. At 14 days of age, chicks in NC group and 3 experimental groups were chal-lenged with 5×104 Eimeria tenella oocysts per bird by oral, whereas chicks in BC group administered by perfu-sion with the same amount of saline. The experimental period lasted for 42 days containing 2 periods of early period (1 to 21 days of age) and later period (22 to 42 days of age). The results showed as follows:1) com-pared with BC group, the average daily gain ( ADG) of broiler chicks during all experimental stages in NC group was significantly decreased and the ratio of feed to gain ( F/G) during 22 to 42 and 1 to 42 days of age was significantly increased ( P<0.05) . Dietary supplemented with 7.5 and 15.0 mg/kg GPC significantly inhib-ited the ADG decrease and F/G increase induced by Eimeria tenella challenged during 22 to 42 and 1 to 42 days of age ( P<0.05) . Dietary supplemented with 30.0 mg/kg GPC significantly decreased the ADG and av-erage daily feed intake ( ADFI) during 22 to 42 days of age and ADG during 1 to 42 days of age ( P<0.05) . 2) Compared with NC group, dietary supplemented with GPC significantly decreased cecal lesion score, bloody stool piles and the number of coccidial oocysts excretion (P<0.05). 3) Dietary supplemented with GPC significantly inhibited the increase of the spleen index of broiler chicks induced by Eimeria tenella chal-lenged (P<0.05). T lymphocyte transformation rate (TLR) was significantly increased by Eimeria tenella challenged ( P<0. 05 ) , and dietary supplemented with 7. 5 and 15. 0 mg/kg GPC further increased it ( P>0.05). Dietary supplemented with 7.5 and 15.0 mg/kg GPC increased the B lymphocyte transformation rate ( BLR) ( P>0.05) . The γδT lymphocytes percentage in peripheral blood was significantly increased by Eime-ria tenella challenged (P<0.05), and dietary supplemented with 15.0 mg/kg GPC further increased it (P>0.05) . 4) Compared with NC group, the ileum intraepithelial lymphocytes ( IEL) number and caecal mucosa secretory IgA ( sIgA) level were significantly increased by Eimeria tenella challenged ( P<0.05) , and dietary supplemented with 7.5 and 15.0 mg/kg GPC further increased them ( P<0.05) . In conclusion, dietary supple-mented with GPC improves immune function and relieve growth inhibition of broiler chicks challenged with Ei-meria tenella, and the supplementation of 7.5 and 15.0 mg/kg GPC have the better anticoccidial effect.关键词
葡萄原花青素/肉仔鸡/柔嫩艾美尔球虫/生长性能/免疫功能Key words
grape procyanidins/broiler chicks/Eimeria tenella/growth performance/immune function分类
农业科技引用本文复制引用
杨金玉,张海军,王晶,岳洪源,武书庚,齐广海,官堃,GERRITS Gert Jan..葡萄原花青素对感染柔嫩艾美尔球虫肉仔鸡生长性能和免疫功能的影响[J].动物营养学报,2016,28(9):2802-2811,10.基金项目
国家自然科学基金(31272456) （31272456）
家禽产业技术体系北京市创新团队项目(CARS-PSTP) （CARS-PSTP）
国家科技支撑计划(2011BAD26B04) （2011BAD26B04）
