动物营养学报2016，Vol.28Issue(9)：2911-2919,9.DOI:10.3969/j.issn.1006-267x.2016.09.030
不同粗饲料条件下梅花鹿瘤胃甲烷菌结构的比较分析
Comparative Analysis of Methanogen Community in Rumen of Sika Deer (Cervus nippon) under Different Forages
摘要
Abstract
The objective of present study was to compare the methanogen community in the rumen of sika deer ( Cervus nippon) fed three common forages using the high throughput sequencing technology. Three 2-year-old male adult Sika deers with permanent ruminal cannulas were used as experimental animal in a 3×3 Latin square design, and their fed diets with oak with leaf (OL group), corn stover (CS group) and corn silage (CI group) as main forage, respectively. After one week of adaption to the diets, sika deer received each diet for 4 weeks. The primers A519F and A976R were used to amplify the V3 to V4 regions of the methanogen 16S rRNA gene. The amplicon was then sequenced on the Illumina MiSeq PE250 platform. The results showed as follows: a total of 600 352 high quality methanogen 16S rRNA gene sequences were obtained from 9 samples. These sequences were classified into 111 operational taxonomic units ( OTU) based on 97% sequence similari-ty. Coverage index showed that 99% of the methanogen species were represented in any given rumen sample. The results of taxonomic analysis showed that Methanobrevibacter spp. [OL group: (97.80±6.00)%; CS group: (97.10±1.50)%; CI group: (87.50±5.50)%] was the dominant methanogen in the rumen of sika deer. However, the distribution of methanogen at species level was different among the three groups. The rela-tive abundance of Methanosphaera stadtmanae in the CI group was significantly higher than that in the OL and CS groups ( P<0.05) . The relative abundance of Methanobrevibacter millerae was increased in the OL and CI groups compared with the CS group ( P>0.05) . The relative abundance of Methanobrevibacter boviskoreani in the OL and CS groups was higher than that in the CI group ( P>0.05) . While, the relative abundance of Meth-anobrevibacter olleyae in the CS and CI groups was increased compared with the OL group ( P>0.05) . These results suggest that Methanobrevibacter spp. is the dominant methanogen in rumen of sika deer.关键词
梅花鹿/甲烷菌/Methanobrevibacter spp./粗饲料Key words
sika deer/methanogen/Methanobrevibacter spp./forage分类
农业科技引用本文复制引用
李志鹏,刘晗璐,司华哲,鲍坤,李光玉..不同粗饲料条件下梅花鹿瘤胃甲烷菌结构的比较分析[J].动物营养学报,2016,28(9):2911-2919,9.基金项目
国家自然科学基金(31501984) （31501984）
吉林省重大科技攻关专项(20140203018NY) （20140203018NY）
