动物营养学报2016，Vol.28Issue(9)：2977-2986,10.DOI:10.3969/j.issn.1006-267x.2016.09.038
云纹石斑鱼幼鱼对高能低氮饲料的适应性
Adaptation of Juvenile Epinephelus moara Fed Diets with High Energy and Low Nitrogen
摘要
Abstract
A 9-week trial was carried out to assess the adaptation of juvenile Epinephelus moara fed diets with high energy and low nitrogen. Nine practical diets were formulated to contain three protein levels [ 35%(P35), 40%(P40) and 45%(P45)] and three lipid levels [9% (L9), 12% (L12) and 15%(L15)] by two-factor experimental design, and in turn marked P35L9, P35L12, P35L15, P40L9, P40L12, P40L15, P45L9, P45L12 and P45L15. Eight hundred and ten juvenile Epinephelus moara with the average initial body weight of 27.09 g were randomly divided in 9 groups with 3 replicates, and each replicate had 30 fish. After feeding for 9 weeks, the growth performance, muscle nutritive composition and serum biochemical parameters of juvenile Epinephelus moara were measured. The results showed as follows: 1) weight gain rate ( WGR) and specific growth rate ( SGR) were firstly increased and then decreased with the increase of dietary protein and lipid levels, and significant interactions between dietary protein and lipid levels were observed in WGR and SGR ( P<0.05) . The highest values of WGR and SGR were all found in P40L12 group which were 184.59%and 1.49%/d, respectively. Feed conversion ratio (FCR), protein efficiency ratio (PER) and feed intake ( FI) reduced gradually with the increase of dietary protein and lipid levels, and significant interactions between dietary protein and lipid levels were observed in them ( P<0.05) . Survival rate showed no significant difference among 9 groups ( P>0.05) . 2) Muscle crude lipid content was significantly affected by dietary lipid level ( P<0.05) , and the highest value was observed in L15 group;dietary protein, lipid levels and their interaction had no significant effects on the contents of crude protein, moisture and ash of muscle (P>0.05). 3) serum glu-tamic oxalacetic transaminase ( GOT) activity was increased with increase dietary lipid level, and the highest value (121.98 U/L) appeared in L15 group, which had significant difference compared with L9 group ( P<0.05); serum glutamic pyruvic transaminase ( GPT) activity was increased with increase dietary protein level, and the highest value (89.79 U/L) appeared in P45 group;serum total cholesterol content was positively cor-related with dietary lipid level ( P<0.05) , whereas it was not significantly affected by dietary protein level ( P>0.05); serum urea nitrogen ( UN) content was negatively correlated with dietary protein level ( P<0.05) , and it had the highest value (1.97 mmol/L) in P35 group, which significantly higher than that in other protein lev-el groups ( P<0.05); dietary protein, lipid levels and their interaction had no significant differences on serum alkaline phosphatase ( AKP) activity and the contents of triglyceride ( TG ) , high-density lipoprotein ( HDL-C) and low-density lipoprotein ( LDL-C) ( P>0.05) . According to these results, juvenile Epinephelus moara fed the diet with 40% protein level and 12% lipid level has the best growth effect, and the utilization ratio of feed is higher under the condition of this experiment.关键词
云纹石斑鱼幼鱼/高能低氮/生长/肌肉营养组成/血清生化指标Key words
juvenile Epinephelus moara/high energy and low nitrogen/growth/muscle nutritive composi-tion/serum biochemical parameters分类
农业科技引用本文复制引用
张廷廷,陈超,邵彦翔,李炎璐,于欢欢,彭士明,杨传军..云纹石斑鱼幼鱼对高能低氮饲料的适应性[J].动物营养学报,2016,28(9):2977-2986,10.基金项目
科技部国际合作项目(2012DFA30360) （2012DFA30360）
农业部东海与远洋渔业资源开发利用重点实验室开放课题资助 （）
