医学分子生物学杂志2016，Vol.13Issue(5)：254-257,4.DOI:10.3870/j.issn.1672-8009.2016.05.002
DKK3基因在心力衰竭家兔心脏中的表达改变研究
Expression of DKK3 in the Heart of Rabbits with Heart Failure
摘要
Abstract
Objective To establish the heart failure model of rabbits by increasing the cardiac preload and afterload and to examine the role of DKK3 in the heart failure by detecting the expres⁃sion change of DKK3. Methods Twenty Japanese white rabbits were randomly divided into the con⁃trol group and the heart failure group. The rabbit aortic valve was punctured to cause reflux and the abdominal aorta was narrowed 2 weeks later in the heart failure group. Animals in the control group only underwent sham operation. After two months, the structure and function of the heart were meas⁃ured, the myocardial cell size and collagen reconstruction were evaluated by hematoxylin and eosin (HE) staining and picrosirius red (PSR) staining, and the changes of DKK3 were detected by immunofluorescence. Results The ejection fraction was significantly decreased, the heart weight, cell size and collagens were markedly increased and the expression of DKK3 was obviously decreased in the heart failure group compared with the control group. Conclusion The DKK3 expression is decreased in heart failure, which weakens the protective effect of DKK3 on cardiac remodeling.关键词
心力衰竭/心脏重构/DKK3基因Key words
heart failure/remodeling/DKK3分类
医药卫生引用本文复制引用
张艳,杨波,张炳山,武欣迎,王澈,梁锦军,李海涛,包明威..DKK3基因在心力衰竭家兔心脏中的表达改变研究[J].医学分子生物学杂志,2016,13(5):254-257,4.基金项目
国家自然科学基金（ No．81200071），湖北省自然科学基金（No．2014 CFA061，2014 CFB739），武汉大学2013年实验室建设项目（No．363004），海南省自然科学基金创新研究团队项目（No．2016 CXTD012）This work was supported by the grants from the National Natural Science Foundation of China （ No．81200071）， the Natural Science Foundation of Hubei Province （No．2014 CFA061， No．2014 CFB739）， the Labo-ratory Construction Project of Wuhan University in 2013（ No．363004）， and Hainan Provincial Innovative Research Team Project （）
