华北农学报2016，Vol.31Issue(4)：126-129,4.DOI:10.7668/hbnxb.2016.04.021
小麦×玉米杂交双单倍体后代的 HMW-GS组成和易位分析
Analysis of HMW-GS and Translocation Line in Progenies of Doubled Haploid from Wheat ×Maize Cross
摘要
Abstract
To improve the efficiency of high quality wheat breeding program,clarify the composition of HMW-GS and Gliadin in the early generation,296 lines from the wheat maize cross were analyzed useing SDS-PAGE meth-od in this experiment,along with this Gliadin were analyzed useing A-PAGE method.A statistics showed that female parent Heng 09-6324 carried null,7 +9,2 +12 subunits,and were 1 BL /1 RS translocation lines with Sec-1 charac-teristic band.Male parent Shiluan 02-1 carried 1 ,7 +9,5 +10 subunits,and were non 1 BL /1 RS translocation.Six types of HMW-GS all originated from parents were identified from 296 DH lines.The frequency of subunits 5 +10 which positively related to bread baking quality was 35.13%.A statistics showed that in the A-PAGE the type of translocation of the DH lines accounted for 54.05%,and the percent of lines with subunits 1 and 5 +10,but non Sec-1 translocation were 15.20%,these lines could be used as new germplasm for quality breeding.The paper indi-cated that that the method of half-seed SDS-PAGE and A-PAGE which could be used to identify and select excellent plants in early generation breeding effectively.关键词
小麦/玉米/双单倍体/半粒电泳法/HMW-GS/黑麦碱Key words
Wheat/Maize/Doubled haploid/Half-seed electrophoresis/HMW-GS/Secalin分类
农业科技引用本文复制引用
庞建周,王雪征,茜晓哲,王晨阳,陈淑萍..小麦×玉米杂交双单倍体后代的 HMW-GS组成和易位分析[J].华北农学报,2016,31(4):126-129,4.基金项目
河北省农林科学院前期预备项目（A2015040301）；河北省农林科学院旱作农业研究所青年基金项目 （）
