海洋科学2016，Vol.40Issue(7)：8-16,9.DOI:10.11759/hykx20150929001
三亚天涯海角潮间带趋磁细菌特性
Characterization of magnetotactic bacteria found in the in-tertidal zone of Tianyahaijiao, Sanya, China
摘要
Abstract
A quantity of magnetotactic bacteria (MTB) were found in the intertidal zone of Tianyahaijiao, Sanya, China, where the maximum abundance can reach up to 100 ind./cm3. Transmission electron microscopy (TEM) re-vealed that most of the MTB were magnetotactic cocci, containing magnetosomes, which were prismatic mineral crystals, mostly organized in chain(s) (one or two chains), while some were in cluster. High-resolution Transmission Electron Microscopy (HRTEM) and Energy Dispersive X-ray Spectroscopy (EDXA) showed that the magnetosomes contained magnetite. It was estimated that the percentage of Fe found in the MTB was 0.70%~7.53% (average 2.05%). Phylogenetic analyses, based on 16S rRNA gene sequences, revealed that 16 sequences of MTB belonged to 9 operational taxonomic units (OTUs) and were affiliated to the class Alphaproteobacteria, except that one OTU was affiliated to the classGammaproteobacteria. Eight OTUs shared < 97% 16S rRNA gene sequence similarity with the nearest known sequences, in which 5 OTUs shared lower than 93% similarity. These results indicate that the MTB resources in Tianyahaijiao were considerable.关键词
潮间带/趋磁细菌/多样性/磁小体/16S rRNA基因Key words
Intertidal zone/magnetotactic bacteria/diversity/magnetosome/16S rRNA gene分类
生物科学引用本文复制引用
徐丛,滕兆洁,肖天,陈海涛,张文燕,陈一然,董逸,潘红苗,杜海舰,王明玲,刘佳..三亚天涯海角潮间带趋磁细菌特性[J].海洋科学,2016,40(7):8-16,9.基金项目
国家自然科学基金项目(41276170,41206150,41330962) （41276170,41206150,41330962）
三亚市院地科技合作项目(2014YD02) （2014YD02）
国家基金委-山东省联合基金项目“海洋生态与环境科学”(U1406403)@@@@National Natural Science Foundation of China, No.41276170, No.41206150, No.41330962 （U1406403）
Project of Academy Locality Science and Technology Cooperation of Sanya City, China, No.2014YD02 （）
NSFC-Shandong Joint Fund “Marine Ecology & Environmental Sciences”, No. U1406403 （）
