海洋科学2016，Vol.40Issue(7)：17-22,6.DOI:10.11759/hykx20140323003
MreB在钝顶螺旋藻中的表达和定位
Expression and Localization of MreB in Spirulina platensis
摘要
Abstract
To explore the function of MreB in morphogenesis of Spirulina platensis, we clonedmreBgene into ex-pression vector pGEX-6P-1 and expressed GST-tagged MreB inEscherichia coli. The purified MreB protein was used to immunize mice to prepare polyclonal antibodies. No obvious differential expression of MreB was detected by western blot analysis between the spiral filaments and linear forms ofSpirulina platensis. Immunofluorescence analysis showed that MreB was distributed as a ring or double helix under the cell membrane. These results showed that MreB might not directly regulate the shapes of the cell. The helical MreB cytoskeletal protein directs the syn-thesis of lateral peptidoglycan by providing a track, therefore, it may affect the morphogenesis ofSpirulina platen-sisby the asymmetric distribution.关键词
螺旋形藻丝体/直线形藻丝体/细胞骨架蛋白Key words
spiral filament/linear filament/Spirulina platensis/cytoskeletal protein分类
农业科技引用本文复制引用
吴娟,金晨钟,胡一鸿,胡军和,竺锡武,徐虹..MreB在钝顶螺旋藻中的表达和定位[J].海洋科学,2016,40(7):17-22,6.基金项目
国家自然科学基金项目(40306024,31301978) （40306024,31301978）
湖南农田杂草防控协同创新中心资助项目@@@@National Natural Science Foundation of China, No.40306024, No.31301978 （）
Hunan Provincial Collaborative Innovation Center for Farmland Weeds Control （）
