海洋科学2016，Vol.40Issue(7)：23-32,10.DOI:10.11759/hykx20151010003
一株海绵附生芽孢杆菌的抗硅藻附着活性成分的分离鉴定
Isolation and identification of active compounds inhibiting diatom settlements using sponge-associated Bacillus sp.
摘要
Abstract
In order to search for natural antifouling compounds, the compounds inhibiting diatom settlements were purified from the metabolites of a sponge-associated Bacillus sp. UST050418-715 via purification of organic solvent extraction and semipreparative high-performance liquid chromatography (HPLC) using antidiatom- settlement assays. The structures of active compounds were analyzed via gas chromatography–mass spectroscopy (GC–MS) and1H nuclear magnetic resonance (NMR). Consequently, seven active cyclic dipeptides in addition to dozens of fractions were isolated and purified. The seven cyclic dipeptides were identified as (1) cyclo (L-leu-trans-8- hydroxy-L-pro), (2) cyclo (L-val-L-pro), (3) cyclo (D-pro-L-leu), (4) cyclo (L-pro-D-leu), (5) cyclo (gly-L-pro), (6) cyclo (L-phe-cis-8-hydroxy-D-pro), and (7) cyclo (L-phe-trans-8-hydroxy-L-pro). The results of this study indicate that multiple cyclic dipeptides exist in the metabolites of sponge-associatedBacillussp. UST050418-715 and contribute to the host’s chemical defense.关键词
抗硅藻附着/芽孢杆菌/UST050418-715/环二肽Key words
antidiatom settlement/Bacillus sp./UST050418-715/cyclic dipeptides分类
海洋科学引用本文复制引用
吴珊,俞思羽,姜薇,张立奎,靳翠丽,周晓见..一株海绵附生芽孢杆菌的抗硅藻附着活性成分的分离鉴定[J].海洋科学,2016,40(7):23-32,10.基金项目
国家自然科学基金资助项目(41306131,41106113,41271521) （41306131,41106113,41271521）
教育部科学技术研究重点项目资助项目(211065) （211065）
江苏省自然科学基金项目(BK2012267, BK20130440) （BK2012267, BK20130440）
江苏省教育厅高校自然科学基金资助项目(13KJB180029,15KJB170020)@@@@National Natural Science Foundation of China, No.41306131, No.41106113 and No.41271521 （13KJB180029,15KJB170020）
Key Project of Chinese Ministry of Education, No.211065 （）
Natural Science Foundation Grant of Jiangsu Province, China, No. BK2012267 and No. BK20130440 （）
Natural Science Foundation for College and University of Jiangsu Province, No.13KJB180029 and No.15KJB170020 （）
