海洋科学2016，Vol.40Issue(7)：46-53,8.DOI:10.11759//hykx20141209001
文蛤一种球形病毒的分离纯化及细胞病理变化观察
Purification and ultrastructure of a spherical virus in the cultured hard clam Meretrix meretrix Linnaeus
摘要
Abstract
In order to study the taxonomic status of a spherical virus and its cytopathic effect on the cells ofMere-trix meretrix, transmission electron microscopy (TEM) was used to observe pathological cell changes in the mori-bund clams and the ultrastructure of the virus. The virus particles were first purified by combining differential cen-trifugation, potassium tartrate cushion ultracentrifugation, and CsCl density gradient centrifugation. The results showed that there were a large number of virus particles in the intercellular substance and cytoplasm of the gill, digestive diverticula, mantle, and digestive tract. The virus particles were spherical, approximately 45 to 55 nm in diameter, of a non-envelope structure, and showed no ambit between the core and nucleocapsid surrounding the cytoplasm. Obvious pathological changes caused by the virus were that the endoplasmic reticulum swelled and mostly turned into vesicles, the nuclear chromatin condensed, the mitochondria swelled and dissolved, the muscle fiber disarrayed, and the number of lysosomes increased. The virus particles were purified at a concentration of 150000 g for 20 h, showed isometric symmetry, were approximately 60 to 75 nm in diameter, showed obvious cap-somers on the surface of the virions, and had a buoyant density of 1.554 to 1.582 g/mL on the isopycnic CsCl gra-dient. The results of this paper supply fundamental data for the study ofMeretrix meretrixpathogens.关键词
文蛤(Meretrix meretrix)/球形病毒/分离纯化/细胞病理Key words
Meretrix meretrix Linnaeus/spherical virus/purification/cytopathology分类
农业科技引用本文复制引用
沈辉,万夕和,何培民,姚国兴,陈爱华..文蛤一种球形病毒的分离纯化及细胞病理变化观察[J].海洋科学,2016,40(7):46-53,8.基金项目
国家科技支撑计划课题(2012BAC07B03) （2012BAC07B03）
2014年省属公益院所科研条件与能力建设(BM2014040)@@@@ Key Project of the National Science & Technology Pillar Program, No.2012BAC07B03 （BM2014040）
The Building of Research Conditions and Capacity of Provincial Institute of Public Welfare in 2014, No. BM2014040 （）
