检验医学与临床2016，Vol.13Issue(19)：2733-2735,3.DOI:10.3969/j.issn.1672-9455.2016.19.014
青浦区水产品中副溶血性弧菌污染状况与分子分型特征
Study on the pollution status and molecular characteristics of Vibrio parahaemolyticus
摘要
Abstract
Objective To explore the pollution status and mole cular characteristics of Vibrio parahaemolyticus in retail aquatic products in Qingpu District .Methods Samples of fish ,shrimp and shellfish from the hotel ,farmers market and super market in dif-ferent seasons were collected ,and then Vibrio parahaemolyticus was isolated and identified by M PN method according to the GB 4789 .7 ,the detection of the virulent genes and molecular typing of the isolated strains were completed .Results A total of 68 strains of Vibrio parahaemolyticus were detected among 144 samples of aquatic products ,the positive rate was 47 .00% .The geo-metric mean concentration of Vibrio parahaemolyticus in shrimp ,shellfish and fish were 465 .2 ,291 .0 ,146 .1 MPN /g .All the detec-ted strains didn′t carry the TDH and TRH virulent genes ,68 strains were divided to 63 patterns by pulse alternative field gel elec-trophoresis(PFGE)method ,which showed high polymorphic .Conclusion The Vibrio parahaemolyticus pollution is serious in retail aquatic products in Qingpu District ,the monitoring on preventing the food poisoning and food origin disease caused by Vibrio parah-aemolyticus should be strengthened ,there is no homology relationship among the detected strains .关键词
水产品/副溶血性弧菌/毒力基因/脉冲场凝胶电泳Key words
aquatic product/Vibrio parahaemolyticus/virulence gene/pulse alternative field gel electrophoresis引用本文复制引用
徐秋芳,卢晓芸,陈洪友,施怡茹..青浦区水产品中副溶血性弧菌污染状况与分子分型特征[J].检验医学与临床,2016,13(19):2733-2735,3.基金项目
青浦区卫生和计划生育委员会项目（W2015-18）。 （）
