临床神经外科杂志2016，Vol.13Issue(5)：321-324,4.DOI:10.3969/j.issn.1672-7770.2016.05.001
转录因子 FOXR2对脑胶质瘤细胞增殖的影响
Effects of FOXR2 on proliferation of glioma cells
摘要
Abstract
Objective To evaluate the effects of FOXR2 on proliferation of glioma cells. Methods The FOXR2 gene were cloned by PCR using cDNA of nontumorous tissue as template and constructed over-expression FOXR2 plasmid; Western blot assay was used to detect the levels of FOXR2 in glioma cell lines.We also constructed stable cell lines of down-regulation and over-expression FOXR2 using lentivirus vector system.CCK8 assay was used to assess the effects of FOXR2 on glioma cell proliferation.Results FOXR2 gene was cloned, and the stable glioma cell lines of down-regulation and over-expression FOXR2 were established successfully;The results of CCK8 assay showed that compared with control group, the cell proliferative rate decreased by 45.13%in FOXR2 down-regulation group, while, the cell number increased by 43.98% in FOXR2 over-expression group.Conclusion FOXR2 could significantly promote the proliferation ability of glioma cells .关键词
脑胶质瘤/FOXR2/增殖Key words
glioma/FOXR2/proliferation分类
医药卫生引用本文复制引用
王大成,刘雪娇,刘宁,涂一鸣,种玉龙,刘慧泽,于如同..转录因子 FOXR2对脑胶质瘤细胞增殖的影响[J].临床神经外科杂志,2016,13(5):321-324,4.基金项目
国家自然科学基金 （）
