临床神经外科杂志2016，Vol.13Issue(5)：325-327,331,4.DOI:10.3969/j.issn.1672-7770.2016.05.002
miR-1224-5p在胶质瘤中的表达及其对胶质瘤细胞增殖的影响
Expression of miR-1224-5p in glioma and its effect on proliferation of glioma cells
摘要
Abstract
Objective To study the expression of miR-1224-5p in glioma and its effect on the proliferation of human glioma cells.Methods The expression of miR-1224-5p in glioma tissues of different grades were analyzed within the Chinese Glioma Genome Atlas expression Dataset( CGGA) and were verified by in situ hybridization.The correlation between the expression of miR-1224-5p and the clinical suvival of glioblastoma patients was analyzed.The miR-1224-5p oligonucleotides were transfected into U251 and U87 glioma cells, the effect of up-regulation of miR-1224-5p on cell proliferation was tested by CCK8 assay.Results The expression of miR-1224-5p in human glioma tissue negatively correlated with tumor pathological grade. Low-expression of miR-1224-5p associated with poor survival in glioblastoma patients.Up-regulation of miR-1224-5p suppressed proliferation in glioma cells.Conclusion The expression level of miR-1224-5 p relates with grading and clinlical prognosis in glioma.miR-1224-5p inhibits the proliferation in glioma cells.关键词
胶质瘤/临床预后/非编码RNA/miR-1224-5p/增殖Key words
glioma/clinical survival/noncoding RNA/miR-1224-5p/proliferation分类
医药卫生引用本文复制引用
钱进,徐英纳,栾文康,史岩,尤永平..miR-1224-5p在胶质瘤中的表达及其对胶质瘤细胞增殖的影响[J].临床神经外科杂志,2016,13(5):325-327,331,4.基金项目
国家自然科学基金 （）
