临床神经外科杂志2016，Vol.13Issue(5)：344-347,351,5.DOI:10.3969/j.issn.1672-7770.2016.05.007
再程序化脂肪干细胞移植治疗大鼠脊髓损伤的机制
Mechanism of reprogrammed adipose derived stem cells transplantation promoting ;recovery of injured spinal cord
摘要
Abstract
Objective To investigate the therapeutic effects of reprogrammed adipose derived stem cell ( ADSCs) in a rat model of spinal cord injury ( SCI) .Methods ADSCs from rats were cultured in vitro and then purified and identified, and genetically modified ADSCs with the neurogenin2 ( Ngn2 ) gene.48 adult female Sprague-Dawley rats were randomly assigned to three groups, the control, ADSC, and Ngn2-ADSCs groups.The hind-limb motor function of all rats was recorded using the Basso, Beattie, and Bresnahan ( BBB ) locomotor rating scale for 8 weeks. Subsequently, haematoxylineosin ( HE) staining, immunohistochemistry were performed.Results Ngn2-ADSCs transplantation group in motor function score, glial scar formation after spinal cord injury and pathological changes in the secretion of neurotrophic factor BDNF and VEGF were significantly better than the other groups.Conclusion Ngn2-ADSCs after transplantation can effectively survive and differentiate into nerve cells, inhibit glial scar formation, reduce spinal cord injury voids, increase BDNF and VEGF expression, and to improve recovery of motor function in SCI rats in a more efficient way than that with ADSCs alone.关键词
脂肪干细胞/脊髓损伤/细胞移植Key words
adipose derived stem cell/spinal cord injuries/cell transplantation分类
医药卫生引用本文复制引用
唐琳俊,葛林波,吴卫军,钱腾达,王希,张开鑫,李立新..再程序化脂肪干细胞移植治疗大鼠脊髓损伤的机制[J].临床神经外科杂志,2016,13(5):344-347,351,5.基金项目
国家自然科学基金（81171147）；安徽省铜陵市卫计委科研项目［2016］25；金坛市科技计划项目 （）
