生物医学工程研究2016，Vol.35Issue(3)：168-174,7.
大鼠脑干星形胶质细胞在应激性胃黏膜损伤中的作用
The Effect of Astrocytes in the Brainstem on the Stress -induced Gastric Mucosal Injury in Rats
摘要
Abstract
To investigate the effect of astrocytes in the nucleus of solitary tract (NTS)and dorsal motor nucleus of the vagus (DMV)on the stress -induced gastric mucosal injury in rats.Thirty -six male Wistar rats were randomly divided into the control group and the Restraint water -immersion stress(RWIS)(0.5、1、2、3、5h)groups.Glial fibers acidic protein (GFAP)expression were tested by immunohistochemical staining.Twelve male Wistar rats were randomly divided into the saline control group and L -AA (inhibition of astrocytes)group.1 h after RWIS,the Fos and the GFAP expression and the damage of gastric mucosa were detected. Results showed that compared with the control group,the number of GFAP positive astrocytes in DMV and NTS in the RWIS groups was significantly increased.The GFAP expression peaked at 1 h after the stress and the spatial of GFAP expression were different.L -AA significantly inhibited GFAP and Fos positive expression in the brainstem (NTS and DMV)and reduced gastric mucosal injury. Astrocytes may participate in the gastrointestinal dysfunction and the gastric mucosal damage induced by RWIS through regulating the activation of neurons.关键词
应激/星形胶质细胞/Fos/孤束核/迷走神经背核Key words
Stress/Astrocytes/Fos/Nucleus of solitary tract/Dorsal motor nucleus of the vagus分类
生物科学引用本文复制引用
秦雪,刘真,王璟,仝令印,孙海基..大鼠脑干星形胶质细胞在应激性胃黏膜损伤中的作用[J].生物医学工程研究,2016,35(3):168-174,7.基金项目
国家自然科学基金资助项目（31672286）；山东省自然科学基金资助项目（ZR2013CM010）。 （）
