生物加工过程2016，Vol.14Issue(5)：6-11,6.DOI:10.3969/j.issn.1672-3678.2016.05.002
谷氨酸棒状杆菌发酵生产L 瓜氨酸及发酵条件优化
Optimization of L-citrulline production by Corynebacterium glutamicum
摘要
Abstract
We optimized the fermentation for L-citrulline production by C. glutamicum 366 strain. Mediam was optimized by response surface analysis Design-Expert software. The fermentation conditions were also studied. The optimum medium was:glucose 63. 33 g/L,arginine 196. 96 mg/L,ammonium sulfate 45. 79 g/L,biotin 35. 72 μg/L,K2HPO4·3H2O 1. 0 g/L,KH2PO4 1. 0 g/L,MgSO4·7H2O 0. 25 g/L,MnSO4· H2 O 0. 02 g/L, FeSO4·7H2 O 0. 02 g/L, ZnCl2 1 mg/L, CuSO4 0. 2 mg/L, vitamin B1 200 μg/L and CaCO3 30 g/L.The optimum fermentation conditions were:temperature at 30 ℃,the rotate speed of 200 r/min,initial pH 7. 0.Under this condition,the production of L-citrulline was 14. 96 g/L after 72 h shake flask fermentation,increased by 75. 8% compared to the control.关键词
谷氨酸棒状杆菌/瓜氨酸/发酵Key words
Corynebacterium glutamicum/citrulline/fermentation分类
化学化工引用本文复制引用
丁叶,郝宁,胡洁,谭玉岩,郭格格..谷氨酸棒状杆菌发酵生产L 瓜氨酸及发酵条件优化[J].生物加工过程,2016,14(5):6-11,6.基金项目
国家自然科学基金（21406113）；江苏省自然科学基金（BK20140932）；江苏省产学研前瞻性联合研究项目 （）
