生物加工过程2016，Vol.14Issue(5)：29-32,4.DOI:10.3969/j.issn.1672-3678.2016.05.006
甘蔗渣固态发酵产纤维素酶
Cellulase production by solid-state fermentation of bagasse
摘要
Abstract
Using one-factor-at-a-time experiments, we optimized solid-state fermentation for cellulose production from bagasse and bran by Trichoderma reesei.The optimum culture conditions were as follows:the dry weight of substrate material was 5. 2 g, the ratio of sugarcane bagasse to wheat bran was 7∶3, (NH4)2SO4 was 7. 5 g/L;enzyme inducer ( glucose) was 1. 6 g/L, surfacant ( PEG6000) was 0. 1 g/solid medium,initial pH was 4. 4, the activation spores access quantity numbers was 5 × 105 spores;fermentation temperature 30℃, fermentation time was 120 h.Under these culture conditions,76. 39 IU/g dry medium cellulase was obtained, which was 3. 76 times higher than the 20. 29 IU/g of the frist medium.关键词
甘蔗渣/里氏木霉/固态发酵/纤维素酶Key words
bagass/Trichoderma reesei/solid-state fermentation/cellulase分类
化学化工引用本文复制引用
李煦媛,宋向阳,李鑫,欧阳嘉,范一民..甘蔗渣固态发酵产纤维素酶[J].生物加工过程,2016,14(5):29-32,4.基金项目
国家自然科学基金（30871992）；江苏省科技支撑计划（BE2010732）；江苏高校优势学科建设工程 （）
