生物加工过程2016，Vol.14Issue(5)：45-50,6.DOI:10.3969/j.issn.1672-3678.2016.05.009
内切菊粉酶在大肠杆菌中的高效表达
High level expression of endoinulinase in Escherichia coli
摘要
Abstract
Inulooligosaccharides( IOS) are a kind of water soluble dietary fiber and have wide application. It can be obtained by hydrolyzing inulin with endoinulinase. Endoinulinase gene inu2 was cloned from Aspergillus ficuum and the recombinant was heterogeneously expressed in Escherichia coli BL21(DE3).In order to improve the expression level, native signal peptide was substituted with pelB and omp C then effects on endoinulinase production were investigated. Moreover, the condition for IOS production was optimized by orthogonal design.As a consequence,the highest endoinulinase activity was 75. 22 U/mg by E. coli INU2-A3,with replacement of pelB as its signal peptide.After 24 h under optimal conditions,of 150 g/L inulin, enzyme concentration of 5 U/g inulin,55 ℃ and pH 4. 6,the IOS yield was 94. 41%. The hydrolysis products of the inulin were mainly IOS with DP3-DP7.关键词
内切菊粉酶/信号肽/大肠杆菌/低聚果糖Key words
en doinulinase/signal peptide/Escherichia coli/inulooligosaccharides分类
生物科学引用本文复制引用
吴若凡,马江锋,戴仲雪,王培培,姜岷..内切菊粉酶在大肠杆菌中的高效表达[J].生物加工过程,2016,14(5):45-50,6.基金项目
国家自然科学基金（21390200）；江苏高校优势学科建设工程 （）
