生物技术通报2016，Vol.32Issue(10)：219-226,8.DOI:10.13560/j.cnki.biotech.bull.1985.2016.10.027
黑麦草根际铁载体产生菌 WN-H3的分离鉴定及其产铁载体培养条件的优化
Screening,Identification and Fermentation Condition Optimun of a Siderophore-producing Bacteria WN-H3 from Rhizosphere of Ryegrass
摘要
Abstract
By mean of the chrome azural S(CAS)assay,a high-affinity siderophore- producing bacteria WN-H3 was screened out from 28 bacteria,which were isolated from the ryegrass(Lolium perenne L.)rhizosphere soil. According to morphological,biochemical and physiological characteristics,16S rDNA sequence and phylogenetic analysis,the strain was identified as Pseudomonas vranovensis. The optimal medium components(carbon and nitrogen sources)and growth conditions(temperature,rotation speed and initial pH)for siderphore production by strain WN-H3 were researched by one variable-at-a-time method. The result demonstrated that high siderophore production was yeilded at high siderophore- producing ability of 80.4% under the condition of 10 g/L sucrose,5 g/L yeast extracion,5 g/L NaCl,temperature 28℃,initial pH7.5,180 r/min.关键词
铁载体产生菌/筛选/鉴定/条件优化Key words
siderophore-producing bacteria/screening/identification/condition optimun引用本文复制引用
陈伟,舒健虹,陈莹,曾庆飞,王小利,陆瑞霞,付薇..黑麦草根际铁载体产生菌 WN-H3的分离鉴定及其产铁载体培养条件的优化[J].生物技术通报,2016,32(10):219-226,8.基金项目
贵州省农业科学院研究生科研创新基金项目（黔农科合（创新基金）2011015号） （）
